hPSCreg®
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WTSIi227-A-1

Registration Summary:

WETU LRP2 KO

WTSIi227-A-1

General

Cell Line
hPSCreg name WTSIi227-A-1
Cite as:
WTSIi227-A-1 (RRID:CVCL_VN07)
Alternative name(s)
WETU LRP2 KO
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
Last update 17th July 2019
Notes LRP2 KO cell line
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Provider
Generator Berlin Institute of Health (BIH)

External Databases
BioSamples SAMEA4861651
Cellosaurus CVCL_VN07
Wikidata Q98135099

General Information
Projects
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information
Sex female
Ethnicity White - White British

Phenotype and Disease related information (Donor)
Diseases No disease was diagnosed.

External Databases (Donor)
BioSamples SAMEA2398744

Ethics

Also have a look at the ethics information for the parental line WTSIi227-A .
Alternatives to consent
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?
Constraints for use or distribution

hIPSC Derivation

General
The source cell information can be found in the parental cell line WTSIi227-A.

Reprogramming method
Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other
Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Medium Essential 8™

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
–
SSEA-4
Yes
–
SOX2
Yes
–

Genotyping

Karyotyping (Cell Line)

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Donnai-Barrow syndrome
Synonyms
  • Holmes-Schepens syndrome
  • Facio-oculo-acoustico-renal syndrome
  • Syndrome of ocular and facial anomalies, telecanthus and deafness
  • Diaphragmatic hernia-hypertelorism-myopia-deafness syndrome
  • FOAR syndrome
  • DBS/FOAR syndrome
  • Diaphragmatic hernia-exomphalos-hypertelorism syndrome
  • Diaphragmatic hernia-hypertelorism-myopia-hearing loss syndrome
  • Syndrome of ocular and facial anomalies, telecanthus and hearing loss
show more synonyms
Genetic modifications
LRP2 (target)
Gene knock-out
CRISPR-associated (CRISPR/Cas) System