WETU LRP2 KO
WTSIi227-A-1
General#
Cell Line |
|
| hPSCreg Name | WTSIi227-A-1 |
| Alternative name(s) |
WETU LRP2 KO
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Last update | 26th April 2018 |
| User feedback | |
Provider |
|
| Generator |
Berlin Institute of Health (BIH)
Contact:
Berlin Institute of Health (BIH) |
| Distributors | |
External Databases |
|
| BioSamples | SAMEA4861651 |
| Cellosaurus | CVCL_VN07 |
General Information |
|
| Notes | LRP2 KO cell line |
| Projects | |
| Is the cell line available in principle? | Available with restrictions: Available for non-commercial research |
| Subclone of | |
Donor Information#
General Donor Information |
|
| Sex | female |
| Ethnicity | White - White British |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
External Databases (Donor) |
|
| BioSamples | SAMEA2398744 |
Ethics#
Also have a look at the ethics information for the parental line
WTSIi227-A
.
| Alternatives to consent |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
| Constraints for use or distribution |
hIPSC Derivation#
General |
|
|
The source cell information can be found in the parental cell line
WTSIi227-A.
|
|
Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Sendai virus |
Vector free reprogramming |
|
Other |
|
| Derived under xeno-free conditions |
Unkown |
| Derived under GMP? |
Unkown |
| Available as clinical grade? |
Unkown |
Culture Conditions#
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Medium |
Essential 8™
|
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| SSEA-4 |
Yes |
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| SOX2 |
Yes |
Genotyping#
Karyotyping (Cell Line) |
|
Other Genotyping (Cell Line) |
Genetic Modification#
| Disease/phenotype related modifications |
|
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