HPSI1113i-wetu_2

General#

Cell Line

hPSCreg Name WTSIi227-A
Alternative name(s)
HPSI1113i-wetu_2
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 9th May 2018
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Provider

Generator Wellcome Trust Sanger Institute (WTSI)
Distributors

External Databases

HipSci HPSI1113i-wetu_2
BioSamples SAMEA2464814
ECACC 77650026
Cellosaurus CVCL_AI06

General Information

Publications
Projects
Is the cell line available in principle? Available with restrictions: Available for non-commercial research
Subclones

Donor Information#

General Donor Information

Sex female
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA2398744

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms
Age of donor (at collection) 55-59
Collected in 2014
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Feeder cells Mouse embryo fibroblast (MEF) feeder cells
Passage method Enzymatically
Collagenase and Dispase
CO2 Concentration 5 %
Medium Other medium:
Base medium:
Main protein source: Knock-out serum replacement

Characterisation#

Analysis of Undifferentiated Cells
Pluripotency Score Novelty Score
50.46 1.372

Certificate of Analysis

Is there a certificate of analysis available?
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI1113i-wetu_2
Number of regions different from primary tissue: 2; Length of differences from primary tissue: 1
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127299
Imputed and phased genotypes
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860612
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860516
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860996
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ447979
GATK haplotype calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ122986
Imputed and phased genotypes
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127534
Genotyping array calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ123053
Splice-aware STAR alignment
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ267003
Abundances of transcripts
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR791594
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859940
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860036
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ122920
mpileup variant calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR946957
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860804
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861092
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860708
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860900
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR847030
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860420
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR946962
Raw sequencing reads
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/
Text file with probe intensities
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861188
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861284
Raw sequencing reads