HPSI1113i-wetu_2
WTSIi227-A
General
Cell Line |
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| hPSCreg name | WTSIi227-A |
| Cite as: | WTSIi227-A (RRID:CVCL_AI06) |
| Alternative name(s) |
HPSI1113i-wetu_2
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
WTSIi227-A-1 (WETU LRP2 KO) |
| Last update | 22nd May 2019 |
| User feedback | |
Provider |
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| Generator | Wellcome Sanger Institute (WTSI) |
| Distributors | |
External Databases |
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| HipSci | HPSI1113i-wetu_2 |
| BioSamples | SAMEA2464814 |
| Cellosaurus | CVCL_AI06 |
| Wikidata | Q54891667 |
General Information |
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| Publications |
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| Projects | |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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| Subclones | |
Donor Information
General Donor Information |
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| Sex | female |
| Age of donor (at collection) | 55-59 |
| Ethnicity | White - White British |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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External Databases (Donor) |
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| BioSamples | SAMEA2398744 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | Yes |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | No |
| If you do not hold the Donor Consent Form, do you know who does? | No |
| Alternatives to consent | |
| Is there other documentation provided to the donor for consenting purposes? | Yes |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | anonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent expressly prevent the derivation of pluripotent stem cells? | No |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
| How may genetic information associated with the cell line be accessed? | Open Access |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | East of England- Cambridge Central Research Ethics Committee. |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? | No |
hIPSC Derivation
General |
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| Source cell type |
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
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| Source cell origin |
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
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| Age of donor (at collection) | 55-59 |
| Collected in | 2014 |
| Source cell line vendor | Cambridge BioResource |
Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Notes on reprogramming vector detection | CytoTune 1 |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Feeder cells |
Mouse embryo fibroblast (MEF) feeder cells |
| Passage method |
Enzymatically
Collagenase and Dispase
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| CO2 Concentration | 5 % |
| Medium |
Other medium:
Base medium:
Main protein source: Knock-out serum replacement |
Characterisation
Analysis of Undifferentiated Cells
| Pluripotency Score | Novelty Score | |
| 50.46 | 1.372 |
Report
HPSI-wetu.pluritest.pluripotency_score.20161010.png
pluripotency image
HPSI-wetu.pluritest.novelty_score.20161010.png
novelty image
Certificate of Analysis |
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| Is there a certificate of analysis available? |
Yes
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Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
No
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Other Genotyping (Cell Line) |
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| Is there genome-wide genotyping or functional data available? |
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI1113i-wetu_2 Number of regions different from primary tissue: 2; Length of differences from primary tissue: 1
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/ Text file with probe intensities |
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