EHTJUi005-A-1

ZB11ALD-S16-5

General#

Cell Line

hPSCreg Name
EHTJUi005-A-1
Alternative name(s)
ZB11ALD-S16-5
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 30th August 2021
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Provider

Generator East Hospital Affiliated to Tongji University (EHTJU)
Owner East Hospital Affiliated to Tongji University (EHTJU)
Distributors
Derivation country China

External Databases

BioSamples SAMEA8904410
CLO CLO_0103324

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: allowed
Subclone of

Donor Information#

General Donor Information

Sex female
Ethnicity Han

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes
Exome sequencing
Health

External Databases (Donor)

BioSamples SAMEA8883269

Ethics#

Also have a look at the ethics information for the parental line EHTJUi005-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

The source cell information can be found in the parental cell line EHTJUi005-A.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
RT-PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Yes
Derived under GMP?
Yes
Available as clinical grade?
No

Culture Conditions#

Surface coating Laminin
Feeder cells
No
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
CO2 Concentration 5 %
Medium Other medium:
Base medium: stemfit
Main protein source:
Serum concentration: %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
OCT4
Yes
SOX2
Yes
TRA-1-60
Yes
Self-renewal
Positive
Endoderm
Unknown
Mesoderm
Unknown
Ectoderm score
Unknown
alkaline phosphatase staining
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
Brachyury
Yes
NCAM
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
β3-tubulin
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX
Passage number: 26
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
Exome sequencing

Genetic Modification#

Disease/phenotype related modifications
Werner Syndrome
Synonyms
  • Werner Syndrome
  • Werner's Syndrome
  • Werner syndrome
  • WS
  • Adult Progeria
  • adult progeria
show more synonyms
Genetic modifications
SUV39H1 (target)
Gene knock-out
Xp11.23
CRISPR-associated (CRISPR/Cas) System