CRISPR/Cas9 mediated generation of a iPSC line EHTJUi005-A-1 with homozygous knockout of the SUV39H1 gene


SUV39H1 is a histone methyltransferase involve numerous biological processes, including of aging, embryo development, tumor growth and mitosis via catalysis of dimethylation and trimethylation of lysine 9 of histone H3. Here we report a human induced pluripotent stem cell line (EHTJUi005-A-1) which is generated from a wildtype human iPSC previously established in our laboratory, and this iPSC has a homozygous knockout of 8 bp in Exon 2 of SUV39H1. This iPSC model provides a valuable resource to study epigenetic regulation in extensive biological processes as mentioned above. Copyright © 2021. Published by Elsevier B.V.

Authors Qiao ZB, Lu JZ, Zhang L, Cao HX, Bai ZH, Qi YY, Zhu HY, Zhang SM, Bao Y, Jia WW, Liu ZM
Journal Stem cell research
Publication Date 2021 Oct;56:102519
PubMed 34464854
DOI 10.1016/j.scr.2021.102519

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