EHTJUi005-A-3

ZB11ALD-L4

General

Cell Line

hPSCreg name EHTJUi005-A-3
Cite as:
EHTJUi005-A-3 (RRID:CVCL_B0JN)
Alternative name(s)
ZB11ALD-L4
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
EHTJUi005-A
(DFGMP-ZB11AL-D)
EHTJUi005-A-1
(ZB11ALD-S16-5)
PUMCHi001-A-1
(IPS34-R-17)
Donor's gene variants:
LMNA
Donor diseases:
familial partial lipodystrophy type 2
WTSIi227-A-1
(WETU LRP2 KO)
Last update 6th September 2021
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Provider

Generator East Hospital Affiliated to Tongji University (EHTJU)
Owner East Hospital Affiliated to Tongji University (EHTJU)
Distributors
Derivation country China

External Databases

BioSamples SAMEA8904409
Cellosaurus CVCL_B0JN
Wikidata Q108820187

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: allowed
Subclone of

Donor Information

General Donor Information

Sex female
Ethnicity Han

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes
Exome sequencing
Health

External Databases (Donor)

BioSamples SAMEA8883269

Ethics

Also have a look at the ethics information for the parental line EHTJUi005-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

The source cell information can be found in the parental cell line EHTJUi005-A.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
RT-PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Yes
Derived under GMP?
Yes
Available as clinical grade?
No

Culture Conditions

Surface coating Laminin
Feeder cells
No
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
CO2 Concentration 5 %
Medium Other medium:
Base medium: STEMFIT
Main protein source:
Serum concentration: %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
OCT4
Yes
SOX2
Yes
TRA-1-60
Yes
Score:
Marker Present Absent
mCpG
OCT4
Self-renewal
Positive
Endoderm
Unknown
Mesoderm
Unknown
Ectoderm score
Unknown
alkaline phosphatase staining
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
Brachyury
Yes
NCAM
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
β3-tubulin
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX
Passage number: 26
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
Exome sequencing

Genetic Modification

Disease/phenotype related modifications
Synonyms
  • HGPS
  • Progeria
Genetic modifications
LMNA (target)
Gene knock-out
1q22
CRISPR-associated (CRISPR/Cas) System