PUMCHi001-A-1

IPS34-R-17

General#

Cell Line

hPSCreg Name PUMCHi001-A-1
Alternative name(s)
IPS34-R-17
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 25th December 2019
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Provider

Generator Peking Union Medical College Hospital (PUMCH)
Owner Peking Union Medical College Hospital (PUMCH)
Derivation country China

External Databases

BioSamples SAMEA6032303

General Information

* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information#

General Donor Information

Sex male
Ethnicity Chinese Han

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
familial partial lipodystrophy type 2
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • reverse partial lipodystrophy
  • familial partial lipodystrophy Dunnigan type
  • familial lipodystrophy of limbs and lower trunk
  • FPLD2
show more synonyms
Genetic variants
LMNA (target)
NM_170707.4:c.1045C>T
Heterozygous

External Databases (Donor)

BioSamples SAMEA6032303

Ethics#

Also have a look at the ethics information for the parental line PUMCHi001-A .
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? Shanghai Gemple Biotechnology Co, LTD, China

hIPSC Derivation#

General

The source cell information can be found in the parental cell line PUMCHi001-A.

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Selection criteria for clones manually picked using a 100 μl tip.
Derived under xeno-free conditions
Unknown
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
SOX2
Yes
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
alpha-SMA
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
Tuj1
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification#

Disease/phenotype related modifications
familial partial lipodystrophy type 2
Synonyms
  • reverse partial lipodystrophy
  • familial partial lipodystrophy Dunnigan type
  • familial lipodystrophy of limbs and lower trunk
  • FPLD2
show more synonyms
Genetic modifications
LMNA (target)
Isogenic modification
NM_170707.4:c.1045C>T
Heterozygous
Repaired