FAMRCi006-A

LMNA T3

General

Cell Line

hPSCreg name FAMRCi006-A
Cite as:
FAMRCi006-A (RRID:CVCL_WP86)
Alternative name(s)
LMNA T3
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
FAMRCi006-B
(LMNA T4)
Donor's gene variants:
LMNA
Donor diseases:
Emery-Dreifuss Muscular Dystrophy
Dilated Cardiomyopathy
FAMRCi005-A
(LMNA B4)
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LMNA
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atrioventricular block
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FAMRCi005-B
(LMNA B5)
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LMNA
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myopathy
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LMNA
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LMNA
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LMNA
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LMNA
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LMNA
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LMNA
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CIRMi976-A
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(CW30306)
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CIRMi981-A
(CW30309)
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CIRMi885-A
(CW30196)
Donor diseases:
Dilated Cardiomyopathy
CIRMi888-A
(CW30199)
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CIRMi894-A
(CW30207)
Donor diseases:
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CIRMi900-A
(CW30213)
Donor diseases:
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Last update 18th April 2022
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Provider

Generator Federal Almazov North-West Medical Research Centre (FAMRC)
Owner Institute of Molecular Biology and Genetics
Distributors
Derivation country Russia

External Databases

Cellosaurus CVCL_WP86
BioSamples SAMEA6275229
Wikidata Q93549770

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Emery-Dreifuss Muscular Dystrophy
  • EDMD
Genetic variants
LMNA (target)
NM_170707.3:c.G1580C
NP_733821.1:p.(Arg527Pro)
Heterozygous
Synonyms
  • Congestive Cardiomyopathy
  • Dilated Cardiomyopathy

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA6275235

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Please provide the contact information Local ethical committee of Almazov National Medical Research Centre
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Local ethical committee of Almazov National Medical Research Centre
Approval number №13/19.06.2014
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Local ethical committee of Almazov National Medical Research Centre
Approval number №13/19.06.2014
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
  • PERIPHERAL BLOOD MONONUCLEAR CELL
  • Peripheral Blood Mononuclear Cell
  • PBMC
Source cell origin
A liquid tissue; its major function is to transport oxygen throughout the body. It also supplies the tissues with nutrients, removes waste products, and contains various components of the immune system defending the body against infection. Several hormones also travel in the blood.
Synonyms
  • Reticuloendothelial System, Blood
  • Peripheral Blood
  • peripheral blood
  • blood
  • Blood
  • Whole Blood
  • BLOOD
  • portion of blood
  • vertebrate blood
show more synonyms

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
RT-PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
ReleSR
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
NANOG
Yes
SSEA-4
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XX
Passage number: 10
Karyotyping method: Array CGH

Other Genotyping (Cell Line)