YCMi009-hDCM009-A

General

Cell Line

hPSCreg name YCMi009-A
Cite as:
YCMi009-A (RRID:CVCL_D0QB)
Alternative name(s)
YCMi009-hDCM009-A
Cell line type Human induced pluripotent stem cell (hiPSC)
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Last update 27th October 2023
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Provider

Generator College of Medicine (YCM)
Owner College of Medicine (YCM)
Distributors
Derivation country South Korea

External Databases

BioSamples SAMEA114466249
Cellosaurus CVCL_D0QB
Wikidata Q123033955

General Information

* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Cardiomyopathy, Congestive
  • Cardiomyopathies, Familial Idiopathic
  • primary dilated cardiomyopathy (disorder)
  • Cardiomyopathies, Congestive
  • Cardiomyopathy, Dilated, CMD1A
  • DCM - Dilated cardiomyopathy
  • Cardiomyopathy, Dilated
  • Dilated Cardiomyopathies
  • Familial Idiopathic Cardiomyopathies
  • COCM - Congestive cardiomyopathy
  • Cardiomyopathy, Dilated, with Conduction Deffect1
  • Congestive cardiomyopathy
  • Familial Idiopathic Cardiomyopathy
  • Idiopathic Cardiomyopathies, Familial
  • Idiopathic Cardiomyopathy, Familial
  • CCM - Congestive cardiomyopathy
  • Cardiomyopathies, Dilated
  • dilated cardiomyopathy
  • Congestive cardiomyopathy (disorder)
  • Congestive dilated cardiomyopathy
  • Primary dilated cardiomyopathy
  • DCM
  • Cardiomyopathy, Familial Idiopathic
  • Cardiomyopathy, Dilated, LMNA
  • Congestive Cardiomyopathies
  • congestive cardiomyopathy
  • familial dilated cardiomyopathy
  • idiopathic dilation cardiomyopathy
  • primary dilated cardiomyopathy
show more synonyms
Genetic variants
cytoband location: 7q32.1
NM_001458.4:c.2003A>G
NP_001449.3:p.Asp668Gly
Heterozygous

External Databases (Donor)

BioSamples SAMEA114494330

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? Yes
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Yonsei University College of Medicine
Approval number 4-2020-0112
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
  • PERIPHERAL BLOOD MONONUCLEAR CELL
  • Peripheral Blood Mononuclear Cell
  • PBMC

Reprogramming method

Vector type Non-integrating
Vector Episomal

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Medium Essential 8™

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
SSEA-4
Yes
Score:
Marker Present Absent
mCpG
OCT4
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
SOX17
Yes
Morphology
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
NKX2-5
Yes
Morphology
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
Rbfox3
Yes
Morphology

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)