SCVIi574C2

General

Cell Line

hPSCreg name SCVIi073-A
Cite as:
SCVIi073-A (RRID:CVCL_C6SK)
Alternative name(s)
SCVIi574C2
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
SCVIi074-A
(SCVIi599C1)
Donor's gene variants:
BAG cochaperone 3
Donor diseases:
Hypertrophic Cardiomyopathy
FAMRCi006-A
(LMNA T3)
Donor's gene variants:
LMNA
Donor diseases:
Emery-Dreifuss Muscular Dystrophy
Dilated Cardiomyopathy
FAMRCi006-B
(LMNA T4)
Donor's gene variants:
LMNA
Donor diseases:
Emery-Dreifuss Muscular Dystrophy
Dilated Cardiomyopathy
USFi003-A
(1AB1)
Donor diseases:
Dilated Cardiomyopathy
CBRCULi016-A-1
(SCN5A p.R219H-14C-ISO)
Donor's gene variants:
SCN5A
Donor diseases:
dilated cardiomyopathy
YCMi009-A
(YCMi009-hDCM009-A)
Donor diseases:
dilated cardiomyopathy
SCVIi050-A
(SCVI2486)
Donor diseases:
Dilated Cardiomyopathy
CIRMi00Q-A
(CW30349)
Donor diseases:
Dilated Cardiomyopathy
CIRMi00R-A
(CW30350)
Donor diseases:
Dilated Cardiomyopathy
CIRMi885-A
(CW30196)
Donor diseases:
Dilated Cardiomyopathy
CIRMi888-A
(CW30199)
Donor diseases:
Dilated Cardiomyopathy
CIRMi894-A
(CW30207)
Donor diseases:
Dilated Cardiomyopathy
CIRMi896-A
(CW30209)
Donor diseases:
Dilated Cardiomyopathy
CIRMi900-A
(CW30213)
Donor diseases:
Dilated Cardiomyopathy
CIRMi903-A
(CW30216)
Donor diseases:
Dilated Cardiomyopathy
CIRMi904-A
(CW30219)
Donor diseases:
Dilated Cardiomyopathy
CIRMi908-A
(CW30223)
Donor diseases:
Dilated Cardiomyopathy
CIRMi912-A
(CW30227)
Donor diseases:
Dilated Cardiomyopathy
CIRMi915-A
(CW30230)
Donor diseases:
Dilated Cardiomyopathy
CIRMi924-A
(CW30240)
Donor diseases:
Dilated Cardiomyopathy
CIRMi926-A
(CW30242)
Donor diseases:
Dilated Cardiomyopathy
CIRMi928-A
(CW30244)
Donor diseases:
Dilated Cardiomyopathy
CIRMi931-A
(CW30247)
Donor diseases:
Dilated Cardiomyopathy
CIRMi932-A
(CW30248)
Donor diseases:
Dilated Cardiomyopathy
CIRMi936-A
(CW30254)
Donor diseases:
Dilated Cardiomyopathy
CIRMi940-A
(CW30259)
Donor diseases:
Dilated Cardiomyopathy
CIRMi941-A
(CW30260)
Donor diseases:
Dilated Cardiomyopathy
CIRMi944-A
(CW30263)
Donor diseases:
Dilated Cardiomyopathy
CIRMi946-A
(CW30265)
Donor diseases:
Dilated Cardiomyopathy
CIRMi948-A
(CW30267)
Donor diseases:
Dilated Cardiomyopathy
CIRMi949-A
(CW30268)
Donor diseases:
Dilated Cardiomyopathy
CIRMi955-A
(CW30275)
Donor diseases:
Dilated Cardiomyopathy
CIRMi967-A
(CW30290)
Donor diseases:
Dilated Cardiomyopathy
CIRMi974-A
(CW30299)
Donor diseases:
Dilated Cardiomyopathy
CIRMi976-A
(CW30302)
Donor diseases:
Dilated Cardiomyopathy
CIRMi978-A
(CW30305)
Donor diseases:
Dilated Cardiomyopathy
CIRMi979-A
(CW30306)
Donor diseases:
Dilated Cardiomyopathy
CIRMi981-A
(CW30309)
Donor diseases:
Dilated Cardiomyopathy
CIRMi987-A
(CW30317)
Donor diseases:
Dilated Cardiomyopathy
CIRMi990-A
(CW30320)
Donor diseases:
Dilated Cardiomyopathy
CIRMi992-A
(CW30322)
Donor diseases:
Dilated Cardiomyopathy
CIRMi995-A
(CW30325)
Donor diseases:
Dilated Cardiomyopathy
CIRMi996-A
(CW30326)
Donor diseases:
Dilated Cardiomyopathy
CIRMi00D-A
(CW30334)
Donor diseases:
Dilated Cardiomyopathy
CIRMi00I-A
(CW30339)
Donor diseases:
Dilated Cardiomyopathy
CIRMi00L-A
(CW30343)
Donor diseases:
Dilated Cardiomyopathy
Last update 23rd January 2023
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Stanford Cardiovascular Institute (SCVI)

External Databases

BioSamples SAMEA112359100
Cellosaurus CVCL_C6SK
Wikidata Q117704859

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex female
Age of donor (at collection) 65-69
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation.
Synonyms
  • Congestive Cardiomyopathy
  • Dilated Cardiomyopathy
Genetic variants
BAG cochaperone 3 (target)
10p14
c.699C>A
p.Tyr233Ter
Heterozygous
SCVIi073-A Sequencing.pdf
Sanger sequencing
BAG cochaperone 3 (target)
10p14
c.784G>A
p.Ala262Thr
Heterozygous
SCVIi073-A Sequencing.pdf
Sanger sequencing
titin (target)
2p12
c.10670dupG
p.Leu3557ThrfsX9
Disease associated phenotypes
  • Dilated cardiomyopathy
Is the medical history available upon request? No
Is clinical information available? H/o non-ischemic cardiomyopathy discovered incidentally in 2014 when patient consulted for symptoms of sinusitis and was found to be tachycardic at 144 bpm and diagnosed with asymptomatic SVT, spontaneously resolved same day.

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA112228227

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: Joseph Wu
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Contact information / weblink https://med.stanford.edu/scvibiobank/people.html
Confirm that consent was obtained by a qualified professional Yes
Has the donor agreed to be re-contacted? Unknown
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? Yes
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Administrative Panel of Human Subjects Research
Approval number 29904
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
  • PERIPHERAL BLOOD MONONUCLEAR CELL
  • Peripheral Blood Mononuclear Cell
  • PBMC
Age of donor (at collection) 65-69

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
RT-PCR
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 22 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: StemMACS
Main protein source: StemMACS Brew
Serum concentration: %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
SOX2
Yes
POU5F1 (OCT-4)
Yes
Morphology pictures
Brightfield 4x
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
Marker Expressed
sox17
Yes
FOXA2
Yes
Mesoderm
Ont Id: UBERON_0000926
Marker Expressed
Brachyury
Yes
TBX6
Yes
Ectoderm
Ont Id: UBERON_0000924
Marker Expressed
OTX2
Yes
PAX6
Yes

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
See below
Passage number: 12
Karyotyping method: KaryoStat+

Other Genotyping (Cell Line)