LCMD-L302P-UCL01C2

General#

Cell Line

hPSCreg Name UCLi006-A
Alternative name(s)
LCMD-L302P-UCL01C2
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 8th December 2017
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Provider

Generator University College London (UCL)
Distributors
Derivation country United Kingdom

External Databases

BioSamples SAMEA104130970
ECACC 66540593
Cellosaurus CVCL_VF11

General Information

Projects
Is the cell line available in principle? Available with restrictions: Available for commercial research

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Congenital muscular dystrophy due to LMNA mutation (primary disease)
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • LMNA-related congenital muscular dystrophy
  • L-CMD
Genetic variants
LMNA (target)
1q21.1-21.2
c.905T>C
p.Leu302Pro
Heterozygous
18551513

External Databases (Donor)

BioSamples SAMEA104130971

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
fibroblast of dermis
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms

Reprogramming method

Vector type Integrating
Vector Virus (Lentivirus)
Genes
Is the used vector excisable?
No
Absence of reprogramming vector(s)?
No
Reprogramming vectors silenced?
Yes
Methods used
Immunostaining, RT-PCR
Vector map

Vector free reprogramming

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
SOX2
Yes
POU5F1 (OCT-4)
Yes
Alkaline Phosphatase
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
Marker Expressed
Alpha FetoProtein
Yes
FOXA2
Yes
GATA Binding Protein 4
Yes
Mesoderm
Ont Id: UBERON_0000926
Marker Expressed
MSX1
Yes
Ectoderm
Ont Id: UBERON_0000924
Marker Expressed
MAP2
Yes
PAX6
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Normal
Karyotyping method: G-Banding

Other Genotyping (Cell Line)