HPSI0816i-fuuy_3

General

Cell Line

hPSCreg name WTSIi658-A
Cite as:
WTSIi658-A (RRID:CVCL_LM39)
Alternative name(s)
HPSI0816i-fuuy_3
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
WTSIi531-A
(HPSI0616i-kayf_5)
Donor diseases:
Spastic paraplegia
WTSIi614-A
(HPSI0616i-rily_3)
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Spastic paraplegia
WTSIi531-B
(HPSI0616i-kayf_6)
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Spastic paraplegia
WTSIi675-A
(HPSI0616i-iuse_5)
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Spastic paraplegia
WTSIi675-B
(HPSI0616i-iuse_6)
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Spastic paraplegia
WTSIi614-B
(HPSI0616i-rily_1)
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Spastic paraplegia
WTSIi479-A
(HPSI0616i-beyk_6)
Donor diseases:
Spastic paraplegia
WTSIi498-A
(HPSI0616i-biln_2)
Donor diseases:
Spastic paraplegia
WTSIi620-A
(HPSI0616i-uegn_3)
Donor diseases:
Spastic paraplegia
WTSIi624-A
(HPSI0616i-uofs_5)
Donor diseases:
Spastic paraplegia
WTSIi648-A
(HPSI0616i-mesv_4)
Donor diseases:
Spastic paraplegia
WTSIi665-A
(HPSI0616i-euia_7)
Donor diseases:
Spastic paraplegia
WTSIi479-B
(HPSI0616i-beyk_2)
Donor diseases:
Spastic paraplegia
WTSIi620-B
(HPSI0616i-uegn_1)
Donor diseases:
Spastic paraplegia
WTSIi648-B
(HPSI0616i-mesv_5)
Donor diseases:
Spastic paraplegia
WTSIi686-B
(HPSI0616i-baxr_6)
Donor diseases:
Spastic paraplegia
WTSIi624-B
(HPSI0616i-uofs_2)
Donor diseases:
Spastic paraplegia
WTSIi498-B
(HPSI0616i-biln_1)
Donor diseases:
Spastic paraplegia
WTSIi686-A
(HPSI0616i-baxr_5)
Donor diseases:
Spastic paraplegia
WTSIi703-B
(HPSI0816i-hizl_4)
Donor diseases:
Spastic paraplegia
WTSIi703-A
(HPSI0816i-hizl_3)
Donor diseases:
Spastic paraplegia
STBCi110-A
(SFC116-03-01)
Donor's gene variants:
CFH
Donor diseases:
type 2 diabetes mellitus
age-related macular degeneration
STBCi071-C
(SFC057-07-02)
Donor diseases:
Alzheimer disease
STBCi090-B
(SFC867-04-12)
Donor diseases:
Parkinson disease
CHDIi002-A
(#2c3, CHDI-90002150)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi006-A
(#6c7, CHDI-90002154)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi008-A
(#8c3, CHDI-90002156)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi011-A
(#11c2, CHDI-90002159)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi017-A
(#17c1, CHDI-90002165)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi018-A
(#18c1, CHDI-90002166)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi019-A
(#19c9, CHDI-90002167)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi021-A
(#21c5, CHDI-90002169)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi028-A
(#28c3, CHDI-90002176)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi035-A
(#108c5, CHDI-90002183)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi036-A
(#110c5, CHDI-90002184)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi038-A
(#112c7, CHDI-90002186)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi040-A
(#115c6, CHDI-90002188)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi045-A
(#121c6, CHDI-90002193)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi046-A
(#122c1, CHDI-90002194)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
STBCi005-B
(SFC833-03-05)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
STBCi005-C
(SFC833-03-14)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
STBCi097-A
(SFC055-04-02)
Donor diseases:
Alzheimer disease
STBCi097-B
(SFC055-04-01)
Donor diseases:
Alzheimer disease
STBCi097-C
(SFC055-04-03)
Donor diseases:
Alzheimer disease
STBCi007-B
(SFC855-03-08)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
STBCi099-A
(SFC059-03-01)
Donor diseases:
Alzheimer disease
STBCi007-C
(SFC855-03-01)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
WTSIi402-A
(HPSI0714i-ouzk_5)
Donor diseases:
Bardet-Biedl syndrome
WTSIi403-A
(HPSI0913i-rufg_4)
Donor diseases:
Bardet-Biedl syndrome
WTSIi405-A
(HPSI0614i-fovu_2)
Donor diseases:
Bardet-Biedl syndrome
Last update 22nd May 2019
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Provider

Generator Wellcome Sanger Institute (WTSI)
Distributors

External Databases

HipSci HPSI0816i-fuuy_3
BioSamples SAMEA104236472
Cellosaurus CVCL_LM39
Wikidata Q54891429

General Information

Projects
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex male
Age of donor (at collection) 60-64
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Synonyms
  • Spastic paraplegia, lower limb

External Databases (Donor)

BioSamples SAMEA7748668

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: HipSci (http://www.hipsci.org/)
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? No
Contact information / weblink
Alternatives to consent
Alternative consent approval number
Is there other documentation provided to the donor for consenting purposes? No
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Details on restriction to research project
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed?
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Please describe how access is provided:
Contact data, institution, or website:
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? No
Name of accrediting authority involved?
Approval number
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? No
Name of accrediting authority involved?
Approval number
Please describe:
Further constraints on use
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?
Constraints for use or distribution

hIPSC Derivation

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Source cell origin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms
Age of donor (at collection) 60-64
Source cell line vendor University College London

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Notes on reprogramming vector detection CytoTune 2

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation

Analysis of Undifferentiated Cells
Pluripotency Score Novelty Score
26.519 1.292

Certificate of Analysis

Is there a certificate of analysis available?
Yes

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0816i-fuuy_3
Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0