General

Cell Line
hPSCreg name	VUi023-A
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	VUi023-A (RRID:CVCL_ZB24)
Alternative name(s)	801.A1
Cell line type	Human induced pluripotent stem cell (hiPSC)
Similar lines	No similar lines found.
Last update	20th November 2020
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Provider
Generator	Vrije Universiteit Amsterdam (VU)
External Databases
Cellosaurus	CVCL_ZB24
BioSamples	SAMEA7762122
Wikidata	Q98134660
General Information
* Is the cell line readily obtainable for third parties?	No

Donor Information

General Donor Information
Sex	male
Phenotype and Disease related information (Donor)
Diseases	No disease was diagnosed.
External Databases (Donor)
BioSamples	SAMEA7762123

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived?	Yes
Was the consent voluntarily given?	Yes
Has the donor been informed that participation will not directly influence their personal treatment?	Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor?	Yes
Do you (Depositor/Provider) hold the original Donor Consent Form?	No
If you do not hold the Donor Consent Form, do you know who does?	Yes
Please provide the contact information	anna.kahler@ki.se
Alternatives to consent are available?	Yes
Alternatives to consent	original swedish instead of english
Alternative consent approval number
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised.	pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells?	Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world?	Yes
How may genetic information associated with the cell line be accessed?	Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample?	No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions?	Yes
Name of accrediting authority involved?	Regional ethics board in Stockholm
Approval number	2011-324-31 and 2012/214-32
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells?	Yes
Name of accrediting authority involved?	Regional ethics board in Stockholm
Approval number	2011-324-31 and 2012/214-32
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General
Source cell type	Fibroblast A connective-tissue cell of mesenchymal origin that secretes proteins and especially molecular collagen from which the extracellular fibrillar matrix of connective tissue forms.
Reprogramming method
Vector type	Non-integrating
Vector	Episomal
Vector free reprogramming
Other
Derived under xeno-free conditions	Unknown
Derived under GMP?	Unknown
Available as clinical grade?	Unknown

Culture Conditions

Medium

TeSR™ E8™

Characterisation

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles
SOX2	Yes
POU5F1 (OCT-4)	Yes
TRA 1-60	Yes
TRA 1-81	Yes

Morphology

Morphology pictures

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	No
Other Genotyping (Cell Line)