Generation of a laminopathies-specific iPSC line EHTJUi005-A-3 with homozygous knockout of the LMNA gene by CRISPR/Cas9 technology
Summary
LAMIN A/C, encoded by the LMNA gene, supports the normal structure of the cell nucleus and regulates the connection between the nucleus and the cytoskeleton as a component of the nucleus envelope. The loss of expression and function of the LMNA gene would lead to the occurrence of congenital muscular dystrophy and Emery-Dreifuss muscular dystrophy which are collectively named as laminopathies. Here, we report a human induced pluripotent stem cell (iPSC) line (EHTJUi005-A-3) generated from a wild iPSC (EHTJUi005-A) with homozygous knockout of the gene LMNA through CRISPR/Cas9. This iPSC line provides a useful research model for studying laminopathies disease. Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Lu JZ, Qiao ZB, Zhang L, Cao HX, Bai ZH, Qi YY, Zhu HY, Chen YQ, Zhang SM, Yan XH, Bao Y, Jia WW, Liu ZM |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2021 Oct;56:102530 |
| PubMed | 34507144 |
| DOI | 10.1016/j.scr.2021.102530 |