Generation of a laminopathies-specific iPSC line EHTJUi005-A-3 with homozygous knockout of the LMNA gene by CRISPR/Cas9 technology


LAMIN A/C, encoded by the LMNA gene, supports the normal structure of the cell nucleus and regulates the connection between the nucleus and the cytoskeleton as a component of the nucleus envelope. The loss of expression and function of the LMNA gene would lead to the occurrence of congenital muscular dystrophy and Emery-Dreifuss muscular dystrophy which are collectively named as laminopathies. Here, we report a human induced pluripotent stem cell (iPSC) line (EHTJUi005-A-3) generated from a wild iPSC (EHTJUi005-A) with homozygous knockout of the gene LMNA through CRISPR/Cas9. This iPSC line provides a useful research model for studying laminopathies disease. Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Lu JZ, Qiao ZB, Zhang L, Cao HX, Bai ZH, Qi YY, Zhu HY, Chen YQ, Zhang SM, Yan XH, Bao Y, Jia WW, Liu ZM
Journal Stem cell research
Publication Date 2021 Oct;56:102530
PubMed 34507144
DOI 10.1016/j.scr.2021.102530

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