Generation of iPSC lines from peripheral blood mononuclear cells from 5 healthy adults


We describe the generation and characterization of 5 human induced pluripotent stem cell (iPSC) lines derived from peripheral blood mononuclear cells (PBMCs) of healthy adult individuals. The PBMCs were reprogrammed using non-integrating Sendai viruses containing the reprogramming factors POU5F1 (OCT4), SOX2, KLF4 and MYC. The iPSC lines exhibited a normal karyotype, expressed pluripotency markers and differentiated into cells representative of the three embryonic germ layers. These iPSC lines can be used as controls in studying disease mechanisms. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Vlahos K, Sourris K, Mayberry R, McDonald P, Bruveris FF, Schiesser JV, Bozaoglu K, Lockhart PJ, Stanley EG, Elefanty AG
Journal Stem cell research
Publication Date 2019 Jan;34:101380
PubMed 30605840
DOI 10.1016/j.scr.2018.101380

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