Generation of iPSC lines from peripheral blood mononuclear cells from 5 healthy adults
Summary
We describe the generation and characterization of 5 human induced pluripotent stem cell (iPSC) lines derived from peripheral blood mononuclear cells (PBMCs) of healthy adult individuals. The PBMCs were reprogrammed using non-integrating Sendai viruses containing the reprogramming factors POU5F1 (OCT4), SOX2, KLF4 and MYC. The iPSC lines exhibited a normal karyotype, expressed pluripotency markers and differentiated into cells representative of the three embryonic germ layers. These iPSC lines can be used as controls in studying disease mechanisms. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Vlahos K, Sourris K, Mayberry R, McDonald P, Bruveris FF, Schiesser JV, Bozaoglu K, Lockhart PJ, Stanley EG, Elefanty AG |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2019 Jan;34:101380 |
| PubMed | 30605840 |
| DOI | 10.1016/j.scr.2018.101380 |