Ma09

General#

Cell Line

hPSCreg Name ACTe002-A
Alternative name(s)
Ma09
Cell line type Human embryonic stem cell (hESC)
Last update 22nd May 2019
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Provider

Generator Advanced Cell Technology, Inc. - Cellular Reprogramming unit (ACT)
Derivation country United States

External Databases

Cellosaurus CVCL_C344

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases

Ethics#

Alternatives to consent are available? Yes
Alternatives to consent

hESC Derivation#

Embryo stage Cleavage (Mitosis)
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No
ZP removal technique Chemical
Cell isolation Mechanical
Cell seeding Single cell and ICM isolated

Culture Conditions#

Feeder cells mouse embryonic fibroblast cell
Cellfinder Ont Id: EFO_0004040
Passage method Enzymatically and Mechanically
CO2 Concentration 5 %
Medium Other medium:
Base medium: DMEM
Main protein source: Fetal bovine serum
Serum concentration: 10 %

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
SSEA-4
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX

Other Genotyping (Cell Line)