Generation of an RNF1-deficient human pluripotent stem cell line using CRISPR/Cas9 technology


RNF1 (RING1A) is a catalytic component of the polycomb repressive complex 1 (PRC1) involved in regulation of, among others, embryonic development and disease progression. However, the exact role of RNF1 in self-renewal and differentiation of human embryonic stem cells (ESCs) remains unknown. Here, we derive one RNF1 knockout human ESC line using CRISPR/Cas9 system. The cell line retains the canonical stem cell morphology and normal karyotype. Moreover, the cell line highly expresses pluripotency genes and has three germ-layer differentiation potential. The RNF1 -/- cell line will be useful for studies on the function and role of RNF1 in human embryonic stem cell fate decisions. Copyright © 2022 The Author(s). Published by Elsevier B.V. All rights reserved.

Authors Zhang D, Zhou M, Zhang Y, Shan Y, Pan G
Journal Stem cell research
Publication Date 2022 May 8;62:102809
PubMed 35567848
DOI 10.1016/j.scr.2022.102809

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