Generation of a gene-edited H9 embryonic stem cell line carrying a DOX-inducible NGN2 expression cassette in the CLYBL locus
Summary
The pro-neural transcription factor neurogenin-2 (NGN2) possesses the ability to rapidly and effectively transform stem cells into fully operational neurons. Here we report the successful generation of a modified H9 human embryonic H9 stem cell line containing a doxycycline (DOX) inducible NGN2 expression construct featuring a floxed Blasticidin/mApple selection module in the safe-harbor locus CLYBL. This cell line retains its pluripotent state in the absence of DOX, yet readily transitions into a neuronal state upon DOX introduction. Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Miellet S, St Clair-Glover M, Maddock M, Dottori M |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2024 Mar;75:103312 |
| PubMed | 38244534 |
| DOI | 10.1016/j.scr.2024.103312 |