Generation of a B2M homozygous knockout human somatic cell nuclear transfer-derived embryonic stem cell line using the CRISPR/Cas9 system


Beta2-microglobulin (B2M) is a subunit of human leukocyte antigen class-I (HLA-I) heterodimer that mediates immune rejection through activation of cytotoxic T cells. B2M binding to HLA-I proteins is essential for functional HLA-I on the cell surface. Here, we generated a B2M homozygous knockout somatic cell nuclear transfer-induced embryonic stem cell (SCNT-ESC) line using CRISPR/Cas9-mediated gene targeting. B2M KO cell line, which does not express HLA-I molecules on cell surface, has pluripotency and differentiation ability to three germ layers. This cell line provides a useful cell source for investigating immunogenicity of allogeneic ESCs and their derivatives for tissue regeneration. Copyright © 2021 The Author(s). Published by Elsevier B.V. All rights reserved.

Authors Lee OH, Lee S, Park M, Moon S, Hwang S, Kim B, Kim CY, Lee DR, Shim SH, Park KH, Chung HM, Choi Y
Journal Stem cell research
Publication Date 2022 Mar;59:102643
PubMed 34971932
DOI 10.1016/j.scr.2021.102643

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