Generation of a B2M homozygous knockout human somatic cell nuclear transfer-derived embryonic stem cell line using the CRISPR/Cas9 system
Summary
Beta2-microglobulin (B2M) is a subunit of human leukocyte antigen class-I (HLA-I) heterodimer that mediates immune rejection through activation of cytotoxic T cells. B2M binding to HLA-I proteins is essential for functional HLA-I on the cell surface. Here, we generated a B2M homozygous knockout somatic cell nuclear transfer-induced embryonic stem cell (SCNT-ESC) line using CRISPR/Cas9-mediated gene targeting. B2M KO cell line, which does not express HLA-I molecules on cell surface, has pluripotency and differentiation ability to three germ layers. This cell line provides a useful cell source for investigating immunogenicity of allogeneic ESCs and their derivatives for tissue regeneration. Copyright © 2021 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Lee OH, Lee S, Park M, Moon S, Hwang S, Kim B, Kim CY, Lee DR, Shim SH, Park KH, Chung HM, Choi Y |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2022 Mar;59:102643 |
| PubMed | 34971932 |
| DOI | 10.1016/j.scr.2021.102643 |