CHAHESe001-A-1

CHA-hES NT6 B2M KO#67

General#

Cell Line

hPSCreg Name
CHAHESe001-A-1
Alternative name(s)
CHA-hES NT6 B2M KO#67
Cell line type Human embryonic stem cell (hESC)
Last update 23rd November 2021
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Provider

Generator Konkuk University (KUSCRB)
Owner CHA University (CHAHES)

External Databases

BioSamples SAMEA11285969
CLO CLO_0103595

General Information

* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
age-related macular degeneration
Synonyms
  • Macular Dystrophy
  • Degeneration, Macular
  • Age Related Maculopathies
  • MACULAR DEGENERATION NOS
  • Age related maculopathy
  • Age Related Macular Degeneration
  • Unspecified senile macular degeneration
  • Maculopathies, Age Related
  • MACULOPATHY AGE RELAT
  • AAMD - Age related macular degeneration
  • Maculopathies, Age-Related
  • Macular Dystrophies
  • ARMD
  • Amended
  • Dystrophies, Macular
  • Age-Related Maculopathy
  • Senile macular degeneration
  • SMD - Senile macular degeneration
  • Macular degeneration (senile) of retina, unspecified
  • Macular Degeneration, Age-Related
  • Degeneration, Age-Related Macular
  • Macular degeneration (disorder)
  • Macular Degenerations
  • Degenerations, Age-Related Macular
  • AGE RELAT MACULOPATHIES
  • Macular Degenerations, Age-Related
  • Maculopathy, Age-Related
  • AMD - Age-related macular degeneration
  • Maculopathy, Age Related
  • Dystrophy, Macular
  • Degenerations, Macular
  • Senile macular retinal degeneration
  • Age-related macular degeneration (disorder)
  • Age related macular degeneration (disorder) [Ambiguous]
  • AGE RELAT MACULOPATHY
  • MACULOPATHIES AGE RELAT
  • Age-Related Macular Degenerations
  • Age-Related Maculopathies
  • Senile macular degeneration of retina
  • ARMD - Age-related macular degeneration
  • Macular Degeneration
  • macular degeneration, age-related
  • AMD
  • age-related macular degeneration
  • age related maculopathy
  • age related Maculopathies
show more synonyms

External Databases (Donor)

BioSamples SAMEA11039299

Ethics#

Also have a look at the ethics information for the parental line CHAHESe001-A .

hESC Derivation#

The source cell information can be found in the parental cell line CHAHESe001-A.

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
ReLeSR
CO2 Concentration 5 %
Medium mTeSR™ Plus
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
FOXA1
Yes
GATA3
Yes
GATA6
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
CDX2
Yes
HAND1
Yes
SNAI2
Yes
Ectoderm
Ont Id: UBERON_0000924
Marker Expressed
MAP2
Yes
PAX6
Yes
SOX1
Yes

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification#

Genetic modifications not related to a disease
B2M (target)
Gene knock-out
cytoband location: 15q21.1
CRISPR-associated (CRISPR/Cas) System