CHA-hES NT6 B2M KO#67
CHAHESe001-A-1
General
Cell Line |
|
| hPSCreg name | CHAHESe001-A-1 |
| Cite as: | CHAHESe001-A-1 (RRID:CVCL_B5RQ) |
| Alternative name(s) |
CHA-hES NT6 B2M KO#67
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines |
MUSIi001-A-1 (B2M-KO-SFiPSC5, HLA-I-null SFiPSCs) RCPCMi007-A-1 (IPSFF1S deltab2m) IRFMNi001-B-2 (CIone M CIITAKO-B2MKO) DHMi005-A-8 (L Delta B2M Clone 6) DHMi005-A-9 (L Delta B2M Clone 11) |
| Last update | 3rd January 2022 |
| User feedback | |
Provider |
|
| Generator |
Konkuk University (KUSCRB)
Contact:
CHA University (CHAHES) |
| Owner | CHA University (CHAHES) |
External Databases |
|
| BioSamples | SAMEA11285969 |
| Cellosaurus | CVCL_B5RQ |
| Wikidata | Q111733119 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
Donor Information
General Donor Information |
|
| Sex | female |
Phenotype and Disease related information (Donor) |
|
| Diseases | A disease was diagnosed.
|
External Databases (Donor) |
|
| BioSamples | SAMEA11039299 |
Ethics
Also have a look at the ethics information for the parental line
CHAHESe001-A
.
hESC Derivation
|
The source cell information can be found in the parental cell line
CHAHESe001-A.
|
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzymatically
ReLeSR
|
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ Plus
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
|
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| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
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Differentiation Potency
In vitro directed differentiation
In vitro directed differentiation
Microbiology / Virus Screening |
|
| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46, XX
Karyotyping method:
G-Banding
|
Other Genotyping (Cell Line) |
|
Genetic Modification
| Genetic modifications not related to a disease |
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