CIone M CIITAKO-B2MKO
IRFMNi001-B-2
General
Cell Line |
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| hPSCreg name | IRFMNi001-B-2 |
| Cite as: | IRFMNi001-B-2 (RRID:CVCL_C1TS) |
| Alternative name(s) |
CIone M CIITAKO-B2MKO
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
IRFMNi001-B-1 (Clone XI CIITAKO) IRFMNi001-B (iPS clone XI) DHMi005-A-8 (L Delta B2M Clone 6) DHMi005-A-9 (L Delta B2M Clone 11) RCPCMi007-A-1 (IPSFF1S deltab2m) MUSIi001-A-1 (B2M-KO-SFiPSC5, HLA-I-null SFiPSCs) IRFMNi001-A (iPS clone IV) |
| Last update | 5th August 2022 |
| User feedback | |
Provider |
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| Generator | IRCCS - Istituto di Ricerche Farmacologiche Mario Negri (IRFMN) |
| Derivation country | Italy |
External Databases |
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| BioSamples | SAMEA110465773 |
| Cellosaurus | CVCL_C1TS |
| Wikidata | Q114311766 |
General Information |
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| Publications |
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| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | male |
| Ethnicity | caucasian |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA104008499 |
Ethics
Also have a look at the ethics information for the parental line
IRFMNi001-B
.
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
IRFMNi001-B.
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Reprogramming method |
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| Vector type | None |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method | Enzymatically |
| Medium |
mTeSR™ Plus
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| NANOG |
Yes |
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| SOX2 |
Yes |
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| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46, XY
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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Genetic Modification
| Genetic modifications not related to a disease |
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