RCPCMi007-A-1

IPSFF1S deltab2m

General#

Cell Line

hPSCreg Name
RCPCMi007-A-1
Alternative name(s)
IPSFF1S deltab2m
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 5th July 2021
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Provider

Generator Federal State Budgetary Institution Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency (RCPCM)
Distributors

External Databases

BioSamples SAMEA8944453
Cellosaurus CVCL_A8DK
CLO CLO_0103326

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: allowed
Commercial: allowed
Subclone of

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA8944452

Ethics#

Also have a look at the ethics information for the parental line RCPCMi007-A .
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation#

General

The source cell information can be found in the parental cell line RCPCMi007-A.

Reprogramming method

Vector type None

Vector free reprogramming

Other

Selection criteria for clones cloning on Tesr1 media for 4-5 passages. the main selection criterion morphology and stable karyotype
Derived under xeno-free conditions
Yes
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
TrypLE
CO2 Concentration 5 %
Medium TeSR™ E8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
DPPA-5
Yes
NANOG
Yes
SALL-4
Yes
SOX2
Yes
SSEA-4
Yes
TRA 1-81
Yes
POU5F1 (OCT-4)
Yes
Morphology pictures
фазовый контраст.tif
phase contrast microscopy
Differentiation Potency
Hepatocyte
Ont Id: CL_0000182
In vitro spontaneous differentiation
Endothelial Cell
Ont Id: NCIT_C12865
In vitro directed differentiation
Marker Expressed
CD31
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
CK18 (keratin 18)
Unknown

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX
Passage number: 13
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification#

Genetic modifications not related to a disease
B2M beta-2-microglobulin (target)
Gene knock-out
Chr 15: 44.71 – 44.72 Mb
CRISPR-associated (CRISPR/Cas) System
seq.jpg
Deletion part of deltab2m gene