IRFMNi001-A

iPS clone IV

General#

Cell Line

hPSCreg Name IRFMNi001-A
Alternative name(s)
iPS clone IV
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 8th December 2017
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Provider

Generator IRCCS - Istituto di Ricerche Farmacologiche Mario Negri (IRFMN)
Derivation country Italy

External Databases

BioSamples SAMEA7614418
Cellosaurus CVCL_IT61

General Information

Publications
Is the cell line available in principle?
No

Donor Information#

General Donor Information

Sex male
Ethnicity caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unkown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA104008499

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
fibroblast of dermis
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms
Age of donor (at collection) neonate

Reprogramming method

Vector type Integrating
Vector Virus (Lentivirus)
Genes
Is the used vector excisable?
Yes
Absence of reprogramming vector(s)?
No
Reprogramming vectors silenced?

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating
Passage method Enzymatically
Accutase
Medium Other medium:
Base medium: DMEM/F12
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
bFGF 10 ng/ml

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
TRA 1-81
Yes
TRA 1-60
Yes
SSEA-4
Yes
SSEA-3
Yes
Self-renewal
Positive
Endoderm
Negative
Mesoderm
Negative
Ectoderm score
Negative
Differentiation Potency
AFP
Ont Id: OMIT_0030150
In vitro spontaneous differentiation
Morphology
Cartilage Element
Ont Id: UBERON_0007844
In vivo teratoma
Morphology
Increased Number Of Actin Filament Phenotype
Ont Id: CMPO_0000105
In vitro spontaneous differentiation
Morphology
Tubulin
Ont Id: PR_000028799
In vitro spontaneous differentiation
Morphology

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
karyotype
Karyotyping method: G-Banding

Other Genotyping (Cell Line)