Generation of a homozygous CIITA knockout iPS cell line using the CRISPR-Cas9 system
Summary
Human induced pluripotent stem cells (iPSCs) have great promise in regenerative medicine. However, several limitations, including immune-incompatibility, have raised concerns regarding their clinical application. Recent studies have shown that human iPSCs and their derivatives lose their immunogenicity when major histocompatibility complex (MHC) class I and II genes are inactivated and CD47 is over-expressed. In this study, we used CRISPR-Cas9 technology to generate an isogenic iPSC line with a homozygous frameshift mutation in the MHC II transactivator (CIITA) gene. The CIITA-/- iPSCs exhibit typical morphology of pluripotent cells, normal karyotype, expression of pluripotency markers and differentiation capacity in the three germ layers. Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Romano E, Trionfini P, Giampietro R, Benigni A, Tomasoni S |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2021 Dec;57:102580 |
| PubMed | 34688128 |
| PubMed Central | PMC8665218 |
| DOI | 10.1016/j.scr.2021.102580 |