UMANe002-A-3
General
Cell Line |
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| hPSCreg name | UMANe002-A-3 |
| Cite as: | UMANe002-A-3 (RRID:CVCL_E3RR) |
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines | |
| Last update | 15th December 2023 |
| User feedback | |
Provider |
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| Generator |
Birket Lab (BIR)
Contact:
University of Manchester (UMAN) |
| Owner | University of Manchester (UMAN) |
| Distributors | |
| Derivation country | United Kingdom |
External Databases |
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| BioSamples | SAMEA114526381 |
| Cellosaurus | CVCL_E3RR |
General Information |
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| Publications |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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| Subclone of | |
Donor Information
General Donor Information |
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| Sex | male |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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| Disease associated phenotypes | no phenotypes |
| Is the medical history available upon request? | Yes |
| Is clinical information available? | Yes |
Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Unknown
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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External Databases (Donor) |
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| BioSamples | SAMEA7791418 |
Ethics
Also have a look at the ethics information for the parental line
UMANe002-A
.
| Is there an MTA available for the cell line? | Yes |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hESC Derivation
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The source cell information can be found in the parental cell line
UMANe002-A.
|
Culture Conditions
| Surface coating | Vitronectin |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
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| O2 Concentration | 21 % |
| CO2 Concentration | 5 % |
| Medium |
Essential 8™
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| TRA 1-60 |
Yes |
Score:
| Marker | Present | Absent |
| mCpG | ||
| OCT4 |
Morphology pictures
Morphology
Differentiation Potency
In vitro directed differentiation
Morphology
NKX2-5 and TNNT2_Flow_Cytometry.pdf
Flow Cytometry of TNNT2-PE and NKX2-5-GFP
In vitro spontaneous differentiation
| Marker | Expressed |
| TUBB3 |
Yes |
Morphology
B3-Tubulin-compressed.pdf
BETA3TUB
Microbiology / Virus Screening |
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| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
Normal 46XY
Passage number: 35
Karyotyping method:
Low-pass whole genome sequencing
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Other Genotyping (Cell Line) |
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Genetic Modification
| Genetic modifications not related to a disease |
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