Man-13

General

Cell Line

hPSCreg name UMANe002-A
Cite as:
UMANe002-A (RRID:CVCL_L201)
Alternative name(s)
Man-13
Cell line type Human embryonic stem cell (hESC)
Similar lines
Last update 21st August 2023
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Provider

Generator University of Manchester (UMAN)
Owner University of Manchester (UMAN)
Distributors
Derivation country United Kingdom

External Databases

BioSamples SAMEA7792168
Cellosaurus CVCL_L201
Wikidata Q54903989

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: allowed
Commercial use: not allowed
Subclones

Donor Information

General Donor Information

Sex male
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes
Is the medical history available upon request? Yes
Is clinical information available? Yes

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
Normal Male 46 XY
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA7791418

Ethics

Was the embryo established purely for research purposes? No
Have both parents consented to the use of the embryo for ESC derivation? Yes
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Please provide contact information of the holder of the original Donor Information Sheet. daniel.brison@manchester.ac.uk
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Please provide the contact information St Mary's hospital IVF unit
Alternatives to consent are available? Yes
Alternatives to consent
Alternative consent approval number
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
a public organisation? Yes
a non-profit company? Yes
a for-profit corporation? No
How may genetic information associated with the cell line be accessed? Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent anticipate that the donor will be notified of results or outcomes of any research involving the donated samples or derived cells? No
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? No
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? No
Does consent permit access to medical records of the donor? No
Does consent permit access to any other source of information about the clinical treatment or health of the donor? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? HTA, HFEA, and UK Stem Cell Bank, Central Manchester LREC favourable opinion 03/CM/684
Approval number HTA Licence number: 22627 HFEA Licence number: R0171-4-a. UKSCB approval number: SCSC12-28

hESC Derivation

Date of derivation 2011-09-05
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes
PGD Embryo?
No
Expansion status 2
Stephenson 2007
ICM morphology D
Stephenson 2007
Trophectoderm morphology a
Stephenson 2007
ZP removal technique Chemical
Cell isolation Mechanical
Cell seeding Isolated ICM
Derived under xeno-free conditions?
No
Derivation under GMP?
Yes
Available as clinical grade?
Yes

Culture Conditions

Surface coating Cellstart
Feeder cells Human dermal fibroblast
Cellfinder Ont Id: CELDA_000001419
Passage method Mechanically
O2 Concentration 20 %
CO2 Concentration 5 %
Medium mTeSR™ 2

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
SSEA-1
No
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Data on pluripotency is in Ye et al 2017 DOI 10.1186/s13287-017-0561-y
Differentiation Potency
ESC Derived Cell Line
Ont Id: EFO_0005738
In vitro spontaneous differentiation
Marker Expressed
GATA Binding Protein 6
Yes
FOXA2
Yes
ESC Derived Cell Line
Ont Id: EFO_0005738
In vitro spontaneous differentiation
Marker Expressed
Vimentin
Yes
Actin, Alpha 2, Smooth Muscle, Aorta
Yes
Kidney
Ont Id: UBERON_0002113
In vivo teratoma
Marker Expressed
Hematoxylin and eosin stain
Yes
Smooth Muscle Tissue
Ont Id: UBERON_0001135
In vivo teratoma
Marker Expressed
Hematoxylin and eosin stain
Yes
ESC Derived Cell Line
Ont Id: EFO_0005738
In vitro spontaneous differentiation
Marker Expressed
Tubulin Beta 3 Class III
Yes
Neurofilament, light polypeptide
Yes
Retinal Pigment Epithelium
Ont Id: BTO_0001177
In vivo teratoma
Marker Expressed
Hematoxylin and eosin stain
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Normal 46 XY
Passage number: 32
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
Trnscriptome
Transcriptome as part of a disease modeling study