UMANe002-A-4

IBM13-19

General

Cell Line

hPSCreg name UMANe002-A-4
Cite as:
UMANe002-A-4
Alternative name(s)
IBM13-19
Cell line type Human embryonic stem cell (hESC)
Similar lines No similar lines found.
Last update 16th April 2024
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Provider

Generator C2T-Kimber lab (UMANC)
Owner Professor Sue Kimber - North West Embryonic Stem Cell Centre
Distributors
Derivation country United Kingdom

External Databases

BioSamples SAMEA115495030

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex male
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Is the medical history available upon request? Yes
Is clinical information available? Yes

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA7791418

Ethics

Also have a look at the ethics information for the parental line UMANe002-A .
Is there an MTA available for the cell line? No
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hESC Derivation

The source cell information can be found in the parental cell line UMANe002-A.

Culture Conditions

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

No characterisation data could be found for this subclone. Please open parental cell line UMANe002-A .

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Genetic modifications
HNF1B (target)
Gene knock-out
17q12
This line contains a heterozygous mutation in HNF1B, that causes a frameshift at the beginning of the HNF1B coding sequence, thus being equivalent to a heterozygous knockout.
CRISPR-associated (CRISPR/Cas) System