UMANe002-A-1

General

Cell Line

hPSCreg name UMANe002-A-1
Cite as:
UMANe002-A-1 (RRID:CVCL_D6JQ)
Cell line type Human embryonic stem cell (hESC)
Similar lines
Last update 22nd August 2023
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Provider

Generator Birket Lab (BIR)
Owner University of Manchester (UMAN)
Distributors
Derivation country United Kingdom

External Databases

BioSamples SAMEA114210484
Cellosaurus CVCL_D6JQ

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex male
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes
Is the medical history available upon request? Yes
Is clinical information available? Yes

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA7791418

Ethics

Also have a look at the ethics information for the parental line UMANe002-A .
Is there an MTA available for the cell line? Yes
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hESC Derivation

The source cell information can be found in the parental cell line UMANe002-A.

Culture Conditions

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
Morphology pictures
UMANe002-A-1_morphology.pdf
Typical morphology
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
FOXA2
Yes
HNF4A
Yes
Cardiac Muscle Cell
Ont Id: CL_0000746
In vitro directed differentiation
Marker Expressed
NKX2-5
Yes
Morphology
Neuron
Ont Id: CL_0000540
In vitro spontaneous differentiation
Marker Expressed
TUBB3
Yes
Morphology
UMANe002-A-1_morphology.pdf
Typical morphology

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Normal 46XY
Passage number: 35
Karyotyping method: Low-pass whole genome sequencing

Other Genotyping (Cell Line)

Genetic Modification

Genetic modifications not related to a disease
NKX2-5 (target)
Gene knock-in
5' knock-in of GFP-T2A-NKX2-5 reporter
CRISPR-associated (CRISPR/Cas) System