The generation and validation of two NKX2-5 fluorescent reporter human embryonic stem cell lines: UMANe002-A-1 and UMANe002-A-2
Summary
The transcription factor NKX2-5 is a highly conserved master regulator of heart development which is widely expressed in cardiac progenitors and cardiomyocytes. Fluorescent reporters of NKX2-5 that minimally perturb normal protein expression can enable the identification, quantification and isolation of NKX2-5-expressing cells in a normal physiological state. Here we report the generation of two new hESC lines with eGFP inserted upstream (5') or downstream (3') of NKX2-5, linked by a cleavable T2A peptide. These complementary reporters produce a robust fluorescent signal in cardiac cells and have wide utility particularly for research on developmental biology and disease modelling. Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Douglas M, O'Loughlin C, Lynch AT, Prialgauskaite R, Adamson AD, Dibb KM, Kimber SJ, Birket MJ |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2024 Feb;74:103262 |
| PubMed | 38100908 |
| DOI | 10.1016/j.scr.2023.103262 |