The generation and validation of a dual cardiac HAND1-Tomato NKX2-5-GFP human embryonic stem cell line UMANe002-A-3

Summary

The transcription factor HAND1 is a critical regulator of cardiac development which is expressed in sub-populations of cardiac progenitors and cardiomyocytes. The transcription factor NKX2-5, in contrast, is expressed more widely in cardiac cells. Here we report the generation of a dual reporter hESC line where the expression of these genes can be simultaneously measured, enabling lineage analysis as well as studies of HAND1 and NKX2-5 gene regulation and protein function. This tool will have wide utility particularly for research on developmental biology and disease modelling. Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.

Authors Lynch AT, Douglas M, Kimber SJ, Birket MJ
Journal Stem cell research
Publication Date 2024 Jun;77:103342
PubMed 38460234
DOI 10.1016/j.scr.2024.103342

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