The generation and validation of a dual cardiac HAND1-Tomato NKX2-5-GFP human embryonic stem cell line UMANe002-A-3
Summary
The transcription factor HAND1 is a critical regulator of cardiac development which is expressed in sub-populations of cardiac progenitors and cardiomyocytes. The transcription factor NKX2-5, in contrast, is expressed more widely in cardiac cells. Here we report the generation of a dual reporter hESC line where the expression of these genes can be simultaneously measured, enabling lineage analysis as well as studies of HAND1 and NKX2-5 gene regulation and protein function. This tool will have wide utility particularly for research on developmental biology and disease modelling. Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Lynch AT, Douglas M, Kimber SJ, Birket MJ |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2024 Jun;77:103342 |
| PubMed | 38460234 |
| DOI | 10.1016/j.scr.2024.103342 |