Generation of induced pluripotent stem cell line (UKWNLi008) derived from a patient carrying a c.1678C>G variant in the transient receptor potential cation channel subfamily A member (TRPA1) gene potentially associated with small fiber neuropathy


Using human dermal fibroblasts (hdF) derived from a patient carrying a c.1678C>G variant located in the TRPA1 gene, induced pluripotent stem cells (iPSC) were generated. Cells were reprogrammed via non-modified (NM) RNA-based transfection resulting in three clones. All three clones showed typical embryonic stem cell-like properties including expression of pluripotency markers, morphology, normal karyotype, and potential differentiation in all three germ layers. With this novel cell line, we offer an in vitro option to study TRPA1 gene variant c.1678C>G and its potential involvement in the development of neuropathic pain as a symptom of small fiber neuropathy (SFN). Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved.

Authors Schottmann NM, Klug K, Klopocki E, Üçeyler N
Journal Stem cell research
Publication Date 2023 Jun;69:103094
PubMed 37079968
DOI 10.1016/j.scr.2023.103094

Research Projects

Cell Lines