Establishment of a human iPSC line (MUSIi007-A) from peripheral blood of normal individual using Sendai viral vectors


Human induced pluripotent stem cell (iPSC) line was generated from peripheral blood mononuclear cells (PBMNCs) isolated from a 26-year-old healthy subject to use as a control group for the iPSC line carrying compound heterozygote for mutation in KLF1 gene. The cells were reprogrammed using integration-free method, Sendai viral (SeV) vectors containing KOS, hc-MYC and hKLF4. The established iPSC line (MUSIi007-A) exhibited a normal karyotype, expressed pluripotent markers and displayed in vitro and in vivo differentiation potential into cells of three embryonic germ layers. Resource table. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Potirat P, Wattanapanitch M, Kheolamai P, Issaragrisil S
Journal Stem cell research
Publication Date 2018 Oct;32:43-46
PubMed 30172906
DOI 10.1016/j.scr.2018.08.014

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