JNCSRe002-A

BJNhem20

The cell line is not validated yet.

General

Cell Line

hPSCreg name JNCSRe002-A
Cite as:
JNCSRe002-A (RRID:CVCL_B843)
Alternative name(s)
BJNhem20
Cell line type Human embryonic stem cell (hESC)
Similar lines
JNCSRe002-A-1
(BJNhem20-OCIAD2-CRISPR-33)
JNCSRe002-A-2
(BJNhem20-OCIAD2-CRISPR-40)
JNCSRe002-A-3
(BJNhem20 OCIAD2-OV)
Last update 24th November 2022
User feedback
Written by Indumathi Mariappan on 2022-04-23 11:17:19  
This human ESC line efficiently differentiates into a variety of ocular cell types such as the corneal and lens epithelium, retinal pigmented epithelium and can form optic cups in 3D cultures.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Jawaharlal Nehru Centre for Advanced Scientific Research (JNCSR)
Derivation country India

External Databases

Cellosaurus CVCL_B843
NIHhESC NIHhESC-10-0084
Wikidata Q54797017
BioSamples SAMEA110755671

General Information

Publications
Projects
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: allowed
Commercial use: allowed
Additional restrictions:

Subject to approval from applicable institutional committees, national committees and national agencies and guidelines.

Subclones

Donor Information

General Donor Information

Sex female
Ethnicity Asian Indian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA110751861

Ethics

Was the embryo established purely for research purposes? No
Have both parents consented to the use of the embryo for ESC derivation? No
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Please provide contact information of the holder of the original Donor Information Sheet. Bangalore Assisted Conception Centre
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Please provide the contact information Bangalore Assisted Conception Centre
Alternatives to consent are available? Yes
Alternatives to consent
Alternative consent approval number NIHhESC-10-0084
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Institutional Commitee for Stem Cell Reseaarch and Therapy (IC-SCRT)
Approval number

hESC Derivation

Date of derivation 2007-04-01
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No
ICM morphology C
Stephenson 2007
Trophectoderm morphology b
Stephenson 2007
ZP removal technique Chemical
Cell isolation Immunosurgery
Cell seeding Isolated ICM

Culture Conditions

Feeder cells mouse embryonic fibroblast cell
Cellfinder Ont Id: EFO_0004040
Passage method Mechanically
CO2 Concentration 5 %
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Fetal Calf Serum
Serum concentration: 5 %
Supplements
L-Glutamine
FGF 2

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
DNMT3B
Yes
GDF3
Yes
ZFP42 (REX-1)
Yes
GABRB3
Yes
GCTM2
Yes
NANOG
Yes
SOX2
Yes
TDGF1
Yes
TERF1
Yes
THY1/CD90
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Microbiology / Virus Screening

HIV 1 Negative
Hepatitis B Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX
Passage number: 44

Other Genotyping (Cell Line)