BJNhem20-OCIAD2-CRISPR-40
JNCSRe002-A-2
General
Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | Asian Indian |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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External Databases (Donor) |
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| BioSamples | SAMEA110751861 |
Ethics
Also have a look at the ethics information for the parental line
JNCSRe002-A
.
hESC Derivation
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The source cell information can be found in the parental cell line
JNCSRe002-A.
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Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells | Cellfinder Ont Id: EFO_0004040 |
| Passage method | Mechanically |
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ Plus
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-81 |
Yes |
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| NANOG |
Yes |
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| SOX2 |
Yes |
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| TDGF1 |
Yes |
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Score:
| Marker | Present | Absent |
| mCpG | ||
| OCT4 |
Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
Normal; 46 XX
Passage number: 19
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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Genetic Modification
| Genetic modifications not related to a disease |
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