General

Cell Line
hPSCreg name	JNCSRe002-A-5
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	JNCSRe002-A-5
Alternative name(s)	BJNhem20-TBXT-2A-EGFP
Cell line type	Human embryonic stem cell (hESC)
Similar lines	JNCSRe002-A (BJNhem20) JNCSRe002-A-4 (BJNhem20 ACTB-eGFP) JNCSRe002-A-1 (BJNhem20-OCIAD2-CRISPR-33) JNCSRe002-A-2 (BJNhem20-OCIAD2-CRISPR-40) JNCSRe002-A-3 (BJNhem20 OCIAD2-OV)
Last update	12th May 2026
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Provider
Generator	Jawaharlal Nehru Centre for Advanced Scientific Research (JNCSR) Contact: Jawaharlal Nehru Centre for Advanced Scientific Research (JNCSR)
Owner	Jawaharlal Nehru Centre for Advanced Scientific Research (JNCSR)
Distributors	Jawaharlal Nehru Centre for Advanced Scientific Research (JNCSR)
Derivation country	India
External Databases
BioSamples	SAMEA120300788
General Information
Publications	Inamdar MS et al. Derivation and characterization of two sibling human embryonic stem cell lines from discarded grade III embryos. Stem cells and development. 2009 Apr;18(3):423-33. Patil Shekhar et al. Generation of a Brachyury reporter cell line (BJNhem20 Brachyury (TBXT)-2A-EGFP) in human embryonic stem cells using CRISPR-Cas9 gene targeting. Stem Cell Research. 2026-03-00.
* Is the cell line readily obtainable for third parties?	Yes Research use: allowed Clinical use: not allowed Commercial use: not allowed
Subclone of	JNCSRe002-A

Genetic Modification

Genetic modifications not related to a disease

TBXT (human) (target) Type of modification Gene knock-in Chromosome location 6q27 Transgene EGFP Chromosome location (transgene) NA Free text Targeting at the C-terminus of Endogenous Brachyury/TBXT gene to generate the EGFP reporter line. Delivery method CRISPR-associated (CRISPR/Cas) System

Donor Information

General Donor Information
Sex	female
Ethnicity	Asian Indian
Phenotype and Disease related information (Donor)
Diseases	No disease was diagnosed.
External Databases (Donor)
BioSamples	SAMEA110751861

Ethics

Also have a look at the ethics information for the parental line JNCSRe002-A .

Is there an MTA available for the cell line?	No
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above?	Yes
Constraints for use or distribution	Research use only. Can be distributed only after required approvals and MTA.

hESC Derivation

The source cell information can be found in the parental cell line JNCSRe002-A.

Culture Conditions

Surface coating	Matrigel/Geltrex
Feeder cells	Mouse Embryonic Fibroblast
Passage method	Mechanically
O2 Concentration	0 %
CO2 Concentration	5 %
Medium	mTeSR™ Plus
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?	No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?	No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?	Yes

Characterisation

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles
NANOG	Yes	–	–
SOX2	Yes	–	–
POU5F1 (OCT-4)	Yes	–

Morphology

Morphology pictures

Differentiation Potency

Endoderm

Endoderm
Ont Id: UBERON_0000925

In vitro spontaneous differentiation

Marker	Expressed
Alpha fetoprotein	Yes

Morphology

FLK1 and Hoechst merge

Mesoderm

Mesoderm
Ont Id: UBERON_0000926

In vitro spontaneous differentiation

Marker	Expressed
Kinase insert domain receptor	Yes

Morphology

Ectoderm

Ectoderm
Ont Id: UBERON_0000924

In vitro spontaneous differentiation

Marker	Expressed
Class III beta tubulin	Yes

Morphology

Beta3tubulin

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes Normal; 46 XX 50635907406-Report.pdf Passage number: 32 Karyotyping method: G-Banding
Other Genotyping (Cell Line)