BJNhem20 OCIAD2-OV
JNCSRe002-A-3
General
Cell Line |
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| hPSCreg name | JNCSRe002-A-3 |
| Cite as: | JNCSRe002-A-3 (RRID:CVCL_C6Q8) |
| Alternative name(s) |
BJNhem20 OCIAD2-OV
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines |
JNCSRe002-A (BJNhem20) JNCSRe002-A-1 (BJNhem20-OCIAD2-CRISPR-33) JNCSRe002-A-2 (BJNhem20-OCIAD2-CRISPR-40) |
| Last update | 10th January 2023 |
| User feedback | |
Provider |
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| Generator | Jawaharlal Nehru Centre for Advanced Scientific Research (JNCSR) |
| Owner | Jawaharlal Nehru Centre for Advanced Scientific Research (JNCSR) |
| Distributors | |
| Derivation country | India |
External Databases |
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| BioSamples | SAMEA111441033 |
| Cellosaurus | CVCL_C6Q8 |
| Wikidata | Q117704219 |
General Information |
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| Publications |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: allowed
Commercial use: allowed
Additional restrictions:
Subject to approval from applicable institutional committees, national committees and national agencies and guidelines. |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | Asian Indian |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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| Disease associated phenotypes | no phenotypes |
Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Yes
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External Databases (Donor) |
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| BioSamples | SAMEA110751861 |
Ethics
Also have a look at the ethics information for the parental line
JNCSRe002-A
.
| Is there an MTA available for the cell line? | No |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hESC Derivation
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The source cell information can be found in the parental cell line
JNCSRe002-A.
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Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells | Cellfinder Ont Id: EFO_0004040 |
| Passage method | Mechanically |
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ Plus
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
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| NANOG |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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| TDGF1 |
Yes |
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Score:
| Marker | Present | Absent |
| mCpG | ||
| OCT4 |
Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
Normal; 46 XX
Passage number: 6
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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Genetic Modification
| Genetic modifications not related to a disease |
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