JNCSRe002-A-3

BJNhem20 OCIAD2-OV

General

Cell Line

hPSCreg name JNCSRe002-A-3
Cite as:
JNCSRe002-A-3 (RRID:CVCL_C6Q8)
Alternative name(s)
BJNhem20 OCIAD2-OV
Cell line type Human embryonic stem cell (hESC)
Similar lines
JNCSRe002-A
(BJNhem20)
JNCSRe002-A-1
(BJNhem20-OCIAD2-CRISPR-33)
JNCSRe002-A-2
(BJNhem20-OCIAD2-CRISPR-40)
Last update 10th January 2023
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Provider

Generator Jawaharlal Nehru Centre for Advanced Scientific Research (JNCSR)
Owner Jawaharlal Nehru Centre for Advanced Scientific Research (JNCSR)
Distributors
Derivation country India

External Databases

BioSamples SAMEA111441033
Cellosaurus CVCL_C6Q8
Wikidata Q117704219

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: allowed
Commercial use: allowed
Additional restrictions:

Subject to approval from applicable institutional committees, national committees and national agencies and guidelines.

Subclone of

Donor Information

General Donor Information

Sex female
Ethnicity Asian Indian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes

External Databases (Donor)

BioSamples SAMEA110751861

Ethics

Also have a look at the ethics information for the parental line JNCSRe002-A .
Is there an MTA available for the cell line? No
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hESC Derivation

The source cell information can be found in the parental cell line JNCSRe002-A.

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells Cellfinder Ont Id: EFO_0004040
Passage method Mechanically
CO2 Concentration 5 %
Medium mTeSR™ Plus
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
TRA 1-60
Yes
TRA 1-81
Yes
TDGF1
Yes
Score:
Marker Present Absent
mCpG
OCT4
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
Forkhead box A2
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
Kinase insert domain receptor
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
Class III beta tubulin
Yes

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Normal; 46 XX
Passage number: 6
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Genetic modifications not related to a disease
Transgene expression
4p11
Stable transfection mediated genomic integration of OCIAD2 ORF