The cell line is not validated yet.
JNCSRe002-A-4
General
Cell Line |
|
| hPSCreg name | JNCSRe002-A-4 |
| Cite as: | JNCSRe002-A-4 |
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines | No similar lines found. |
| Last update | 21st May 2025 |
| User feedback | |
Provider |
|
| Generator | Jawaharlal Nehru Centre for Advanced Scientific Research (JNCSR) |
| Owner | Jawaharlal Nehru Centre for Advanced Scientific Research (JNCSR) |
| Distributors | |
| Derivation country | India |
External Databases |
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| BioSamples | SAMEA118336877 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Additional restrictions:
Requires approvals and MTA |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | Asian Indian |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
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External Databases (Donor) |
|
| BioSamples | SAMEA110751861 |
Ethics
Also have a look at the ethics information for the parental line
JNCSRe002-A
.
| Is there an MTA available for the cell line? | No |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | Yes |
| Constraints for use or distribution | Research use only. Can be distributed only after required approvals and MTA. |
hESC Derivation
|
The source cell information can be found in the parental cell line
JNCSRe002-A.
|
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method | Mechanically |
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ Plus
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
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| NANOG |
Yes |
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| TDGF1 |
Yes |
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| POU5F1 (OCT-4) |
Yes |
|
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| SOX2 |
Yes |
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| NANOG |
Yes |
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| TDGF1 |
Yes |
|
Score:
| Marker | Present | Absent |
| mCpG | ||
| OCT4 |
Differentiation Potency
In vitro spontaneous differentiation
| Marker | Expressed |
| Kinase insert domain receptor |
Yes |
| Kinase insert domain receptor |
Yes |
In vitro spontaneous differentiation
| Marker | Expressed |
| Class III beta tubulin |
Yes |
| Class III beta tubulin |
Yes |
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
Normal; 46 XX
Passage number: 20
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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Genetic Modification
| Genetic modifications not related to a disease |
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