BJNhem20-OCIAD2-CRISPR-33
JNCSRe002-A-1
General
Donor Information
General Donor Information |
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Sex | female |
Ethnicity | Asian Indian |
Phenotype and Disease related information (Donor) |
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Diseases | No disease was diagnosed.
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External Databases (Donor) |
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BioSamples | SAMEA110751861 |
Ethics
Also have a look at the ethics information for the parental line
JNCSRe002-A
.
hESC Derivation
The source cell information can be found in the parental cell line
JNCSRe002-A.
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Culture Conditions
Surface coating | Matrigel/Geltrex |
Feeder cells | Cellfinder Ont Id: EFO_0004040 |
Passage method | Mechanically |
CO2 Concentration | 5 % |
Medium |
mTeSR™ Plus
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Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
POU5F1 (OCT-4) |
Yes |
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SSEA-4 |
Yes |
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TRA 1-81 |
Yes |
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NANOG |
Yes |
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SOX2 |
Yes |
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TDGF1 |
Yes |
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Score:
Marker | Present | Absent |
mCpG | ||
OCT4 |
Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
Normal; 46XX
Passage number: 19
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
Genetic Modification
Genetic modifications not related to a disease |
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