JNCSRe002-A-1

BJNhem20-OCIAD2-CRISPR-33

General

Cell Line

hPSCreg Name
JNCSRe002-A-1
Alternative name(s)
BJNhem20-OCIAD2-CRISPR-33
Cell line type Human embryonic stem cell (hESC)
Last update 4th September 2022
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Provider

Generator Jawaharlal Nehru Centre for Advanced Scientific Research (JNCSR)
Owner Bangalore Stem Cell Group
Distributors
Derivation country India

External Databases

BioSamples SAMEA110751570
CLO CLO_0104028

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: allowed
Commercial use: allowed
Additional restrictions:

Subject to approval from applicable institutional committees, national committees and national agencies and guidelines.

Subclone of

Donor Information

General Donor Information

Sex female
Ethnicity Indian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA110751861

Ethics

Also have a look at the ethics information for the parental line JNCSRe002-A .

hESC Derivation

The source cell information can be found in the parental cell line JNCSRe002-A.

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells Cellfinder Ont Id: EFO_0004040
Passage method Mechanically
CO2 Concentration 5 %
Medium mTeSR™ Plus
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-81
Yes
NANOG
Yes
SOX2
Yes
TDGF1
Yes
Score:
Marker Present Absent
mCpG
OCT4
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
Forkhead box A2
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
Kinase insert domain receptor
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
Class III beta tubulin
Yes

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Normal; 46XX
Passage number: 19
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Genetic modifications not related to a disease
OCIAD2 (target)
Gene knock-out
4p11
CRISPR-Cas9 paired nickase mediated targeting of OCIAD2 exon 4 leading to a biallelic knockout line
CRISPR-associated (CRISPR/Cas) System