hiPSC-CD34-GFP-3G6
TMOi001-A-1
General
Cell Line |
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| hPSCreg name | TMOi001-A-1 |
| Cite as: | TMOi001-A-1 (RRID:CVCL_UL03) |
| Alternative name(s) |
hiPSC-CD34-GFP-3G6
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
TMOi001-A (Gibco Episomal iPSC Line; A18945) TMOi001-A-6 (DISC1 Ex2 C4) TMOi001-A-5 (DISC1 Ex2 D5) TMOi001-A-4 (SPG11_HA) |
| Last update | 22nd May 2019 |
| User feedback | |
Provider |
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| Generator | St. Anna Kinderkrebsforschung GmbH (CCRI) |
| Distributors | |
| Derivation country | Austria |
External Databases |
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| BioSamples | SAMEA104619935 |
| Cellosaurus | CVCL_UL03 |
| Wikidata | Q98133482 |
General Information |
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| Publications |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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| Is the medical history available upon request? | No |
| Is clinical information available? | No |
Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Unknown
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA104619829 |
Ethics
Also have a look at the ethics information for the parental line
TMOi001-A
.
| Alternatives to consent | |
| Is there an MTA available for the cell line? | Yes |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | DNA vectors in part from GenScript |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
| Constraints for use or distribution |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
TMOi001-A.
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Episomal |
| Genes | |
| Is reprogramming vector detectable? |
No |
| Methods used |
PCR, RT-PCR
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| Notes on reprogramming vector detection | negative Southern blot |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzymatically
Accutase
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| O2 Concentration | 3 % |
| CO2 Concentration | 5 % |
| Medium |
TeSR™ E8™
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| NANOG |
Yes |
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| SOX2 |
Yes |
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| KLF4 |
Yes |
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Score:
| Marker | Present | Absent |
| mCpG | ||
| OCT4 |
Differentiation Potency
Microbiology / Virus Screening |
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| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46,XX
Karyotyping method:
Q-banding
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Other Genotyping (Cell Line) |
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Genetic Modification
| Genetic modifications not related to a disease |
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