Gibco Episomal iPSC Line; A18945

General#

Cell Line

hPSCreg Name TMOi001-A
Alternative name(s)
Gibco Episomal iPSC Line; A18945
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 8th December 2017
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Provider

Generator Thermo Fisher Scientific (TMO)
Owner Thermo Fisher Scientific (TMO)
Derivation country United States

External Databases

BioSamples SAMEA2253216
Cellosaurus CVCL_RM92

General Information

Notes This iPSC Line is available for purchase: https://www.thermofisher.com/order/catalog/product/A18945?SID=srch-srp-A18945
Publications
Projects
Is the cell line available in principle? Available with restrictions: Available for commercial research
Subclones

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes
Is the medical history available upon request? No
Is clinical information available? No

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA104619829

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
hematopoietic stem cell
A stem cell from which all cells of the lymphoid and myeloid lineages develop, including blood cells and cells of the immune system. Hematopoietic stem cells lack cell markers of effector cells (lin-negative). Lin-negative is defined by lacking one or more of the following cell surface markers: CD2, CD3 epsilon, CD4, CD5 ,CD8 alpha chain, CD11b, CD14, CD19, CD20, CD56, ly6G, ter119.
Synonyms
  • blood forming stem cell
  • HSC
  • hemopoietic stem cell
Source cell origin
umbilical cord blood
blood that remains in the placenta and in the attached umbilical cord after childbirth[WP].
Source cell type (free text) cord blood-derived CD34+ progenitors
Age of donor (at collection) neonate
Source cell line vendor AllCells Emeryville CA

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
No
Methods used
PCR, RT-PCR
Notes on reprogramming vector detection negative Southern blot

Vector free reprogramming

Other

Selection criteria for clones see publication
Derived under xeno-free conditions
No
Derived under GMP?
Unkown
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
Accutase
O2 Concentration 3 %
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
KLF4
Unkown
Transcriptome Characterisation
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Hematopoietic Precursor Cell
Ont Id: CL_0008001
In vivo teratoma
In vitro directed differentiation
Marker Expressed
CD31 (PECAM1)
Yes
CD34
Yes
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage: P30

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX

Other Genotyping (Cell Line)