Generation of two induced pluripotent stem cell lines (TMOi001-A-5, TMOi001-A-6) carrying variants in DISC1 exon 2 using CRISPR/Cas9 gene editing


DISC1 is a scaffold protein involved in key developmental processes such as neuronal migration, differentiation and neurogenesis. Genetic variants of the DISC1 gene have been linked to neuropsychiatric disorders like schizophrenia, bipolar disorder and major depression. Here, we generated two isogenic iPSC lines carrying mutations in DISC1 exon 2 using CRISPR/Cas9 gene editing. Both lines express pluripotency markers, can be differentiated into the three germ layers and present a normal karyotype. The generated iPSC lines can be used to study the implications of DISC1 mutations in the context of neuropsychiatric diseases in vitro. Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Heider J, Sperlich D, Vogel S, Breitmeyer R, Volkmer H
Journal Stem cell research
Publication Date 2022 Sep 20;64:102925
PubMed 36154917
DOI 10.1016/j.scr.2022.102925

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