TMOi001-A-6

DISC1 Ex2 C4

General

Cell Line

hPSCreg Name
TMOi001-A-6
Alternative name(s)
DISC1 Ex2 C4
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 31st August 2022
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Provider

Generator NMI Natural and Medical Sciences Institute at the University of Tübingen (NMI)
Owner NMI Natural and Medical Sciences Institute at the University of Tübingen (NMI)
Derivation country Germany

External Databases

BioSamples SAMEA110360471
CLO CLO_0104065

General Information

Publications
* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes
Is the medical history available upon request? No
Is clinical information available? No

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104619829

Ethics

Also have a look at the ethics information for the parental line TMOi001-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

The source cell information can be found in the parental cell line TMOi001-A.

Reprogramming method

Vector type Non-integrating
Vector Episomal
Methods used
PCR, RT-PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
Accutase
CO2 Concentration 5 %
Medium mTeSR™ Plus
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
SOX2
Yes
TRA 1-60
Yes
SSEA-4
Yes
Differentiation Potency
Endoderm
Ont Id: NCIT_C12706
In vitro directed differentiation
Marker Expressed
SOX17
Yes
Mesoderm
Ont Id: NCIT_C12750
In vitro directed differentiation
Marker Expressed
Brachyury
Yes
Ectoderm
Ont Id: NCRO_0000111
In vitro directed differentiation
Marker Expressed
PAX6
Yes

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX
Karyotyping method: Array CGH

Other Genotyping (Cell Line)

Genetic Modification

Genetic modifications not related to a disease
DISC1 (target)
Variant
1q42.2
NG_011681.2: c.[72210_72226delACTCGAGAGGCCTCTTG] ; [72210_72211insA]
Homozygous