TMOi001-A-4

SPG11_HA

General#

Cell Line

hPSCreg Name TMOi001-A-4
Alternative name(s)
SPG11_HA
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 4th June 2021
Notes endogenously tagged SPG11- human iPSC line generated by CRISPR/Cas9 mediated knock-in of HA tag into the c-terminal domain of the gene sequence.
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Universitätsklinikum Erlangen (UKER)

External Databases

BioSamples SAMEA7807684

General Information

* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Is the medical history available upon request? No
Is clinical information available? No

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
Karyotyping method: Chromosomal microarray analysis with Affymetrix CytoScan HD

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104619829

Ethics#

Also have a look at the ethics information for the parental line TMOi001-A .
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

The source cell information can be found in the parental cell line TMOi001-A.

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
No
Methods used
PCR, RT-PCR
Notes on reprogramming vector detection negative Southern blot

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
SOX17
Yes
Protocol or reference
Endoderm
Ont Id: UBERON_0000925
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
Brachyury
Yes
HAND1
Unknown
Mesoderm
Ont Id: UBERON_0000926
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
sox1
Yes
otx2
Unknown

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Other Genotyping (Cell Line)

Genetic Modification#

Disease/phenotype related modifications
(unknown disease)
Genetic modifications
Genetic modifications not related to a disease
SPG11 (target)
Gene knock-in
15q21
HA tag inserted at C-terminal domain of SPG11
CRISPR-associated (CRISPR/Cas) System