SPG11_HA
TMOi001-A-4
General
Cell Line |
|
| hPSCreg name | TMOi001-A-4 |
| Cite as: | TMOi001-A-4 (RRID:CVCL_A8SW) |
| Alternative name(s) |
SPG11_HA
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
TMOi001-A (Gibco Episomal iPSC Line; A18945) TMOi001-A-1 (hiPSC-CD34-GFP-3G6) TMOi001-A-6 (DISC1 Ex2 C4) TMOi001-A-5 (DISC1 Ex2 D5) |
| Last update | 4th June 2021 |
| Notes | endogenously tagged SPG11- human iPSC line generated by CRISPR/Cas9 mediated knock-in of HA tag into the c-terminal domain of the gene sequence. |
| User feedback | |
Provider |
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| Generator | Universitätsklinikum Erlangen (UKER) |
External Databases |
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| BioSamples | SAMEA7807684 |
| Cellosaurus | CVCL_A8SW |
| Wikidata | Q108821374 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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| Is the medical history available upon request? | No |
| Is clinical information available? | No |
Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Yes
Karyotyping method:
Chromosomal microarray analysis with Affymetrix CytoScan HD
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA104619829 |
Ethics
Also have a look at the ethics information for the parental line
TMOi001-A
.
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
TMOi001-A.
|
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Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Episomal |
| Genes | |
| Is reprogramming vector detectable? |
No |
| Methods used |
PCR, RT-PCR
|
| Notes on reprogramming vector detection | negative Southern blot |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
|
| Medium |
mTeSR™ 1
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
|
Differentiation Potency
In vitro directed differentiation
Protocol or reference
In vitro directed differentiation
Protocol or reference
Microbiology / Virus Screening |
|
| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
|
Other Genotyping (Cell Line) |
Genetic Modification
| Disease/phenotype related modifications |
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| Genetic modifications not related to a disease |
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