A scalable and tunable thermoreversible polymer for 3D human pluripotent stem cell biomanufacturing


Human pluripotent stem cells (hPSCs) are an exciting and promising source to enable cell replacement therapies for a variety of unmet medical needs. Though hPSCs can be successfully derived into numerous physiologically relevant cell types, effective translation to the clinic is limited by challenges in scalable production of high-quality cells, cellular immaturity following the differentiation process, and the use of animal-derived components in culture. To address these limitations, we have developed a fully defined, reproducible, and tunable thermoreversible polymer for high-quality, scalable 3D cell production. Our reproducible synthesis method enables precise control of gelation temperature (24°C-32°C), hydrogel stiffness (100-4000 Pa), and the prevention of any unintended covalent crosslinking. After material optimization, we demonstrated hPSC expansion, pluripotency maintenance, and differentiation into numerous lineages within the hydrogel. Overall, this 3D thermoreversible hydrogel platform has broad applications in scalable, high-quality cell production to overcome the biomanufacturing burden of stem cell therapy. © 2022 The Authors.

Authors Johnson HJ, Chakraborty S, Muckom RJ, Balsara NP, Schaffer DV
Journal iScience
Publication Date 2022 Oct 21;25(10):104971
PubMed 36147944
PubMed Central PMC9485071
DOI 10.1016/j.isci.2022.104971

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