Generate an AZFa deleted human embryonic stem cell line
Summary
Y chromosome deletion and karyotype abnormalities are commonly associated with congenital non-obstructive azoospermia, impairing spermatogenesis. Specifically, the deletion of the Y chromosome Azoospermia factor a (AZFa) has been identified in infertile males with severely impaired spermatogenesis. AZFa, encompassing megabase-scale of the Y chromosome region, poses challenges in modeling AZFa deletion-related male infertility using gene editing tools. Here, we successfully created an AZFa-deleted human embryonic stem cell line utilizing the CRISPR/Cas9 gene editing tool. Our analysis indicates the AZFa-deleted stem cell line holds promise for differentiation into ectoderm, mesoderm, and endoderm, highlighting its potential for further comprehensive study. Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Zhen Y, He Q, Sun C, Ma Y, Li Q, Wen L |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2024 Jun;77:103436 |
| PubMed | 38733811 |
| DOI | 10.1016/j.scr.2024.103436 |