ALSH84C5

General

Cell Line

hPSCreg name NUIGi048-C
Cite as:
NUIGi048-C (RRID:CVCL_YQ92)
Alternative name(s)
ALSH84C5
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
UNAMi001-A
(IFC-UNAM iPD01-S)
Donor diseases:
Parkinson Disease
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Donor diseases:
Joubert syndrome
UNAMi002-A
(IFC-UNAM iPD02-S)
Donor diseases:
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(IFC-UNAM iPD03-PINK1)
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Last update 3rd December 2019
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Provider

Generator National University of Ireland Galway (NUIG)

External Databases

BioSamples SAMEA6276789
Cellosaurus CVCL_YQ92
Wikidata Q98128232

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex male
Age of donor (at collection) 25-29
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is affected.
Synonyms
  • sporadic amyotrophic lateral sclerosis

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
SNP data image
Karyotyping method: Molecular karyotyping by SNP array

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA6276790

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? No
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Research Ethics Committee of Galway University Hospitals
Approval number C.A.750
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Research Ethics Committee of Galway University Hospitals
Approval number C.A.750
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
Dermal fibroblasts are the major cell type in dermis and are commonly accepted as terminally differentiated cells.
Age of donor (at collection) 25-29
Collected in 2012
Passage number reprogrammed P7

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
CO2 Concentration 5 %
Medium Essential 8™ Flex
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
SSEA-4
Yes
Morphology pictures
ALSH84C5P10 10X 03092018.tif
ALSH84C5 MORPHOLOGY
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
SNP data image
Karyotyping method: Molecular karyotyping by SNP array

Other Genotyping (Cell Line)