AMUFAHi002-A

AY20210813

General#

Cell Line

hPSCreg Name
AMUFAHi002-A
Alternative name(s)
AY20210813
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 5th May 2022
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Provider

Generator The First Affiliated Hospital of Anhui Medical University (AMUFAH)

External Databases

BioSamples SAMEA13819042

General Information

* Is the cell line readily obtainable for third parties?
No

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
polycystic ovary syndrome
Synonyms
  • Sclerocystic Ovaries
  • Sclerocystic Ovary Syndrome
  • Ovary, Sclerocystic
  • PCOS - Polycystic ovarian syndrome
  • polycystic ovarian disease
  • polycystic ovaries
  • polycystic ovary syndrome
  • Ovarian Syndromes, Polycystic
  • Stein-Leventhal syndrome
  • Ovary Syndrome, Polycystic
  • multicystic ovaries
  • Polycystic ovarian syndrome
  • Polycystic ovarian disease
  • Ovarian Degeneration, Sclerocystic
  • Stein Leventhal Syndrome
  • PCO - Polycystic ovaries
  • Sclerocystic Ovarian Degeneration
  • Ovaries, Sclerocystic
  • Stein-Leventhal synd.
  • Cystic disease of ovaries
  • PCOD - Polycystic ovarian disease
  • Polycystic ovaries
  • Multicystic ovaries
  • Stein-Leventhal Syndrome
  • polycystic ovary
  • PCOS
  • Syndrome, Polycystic Ovary
  • Ovarian Syndrome, Polycystic
  • Polycystic ovaries (disorder)
  • Syndrome, Stein-Leventhal
  • Sclerocystic Ovary
show more synonyms

External Databases (Donor)

BioSamples SAMEA13819043

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

Source cell type
Peripheral Blood Mononuclear Cell
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
  • PERIPHERAL BLOOD MONONUCLEAR CELL
  • Peripheral Blood Mononuclear Cell
  • Peripheral Blood Mononuclear cells
  • PBMC
show more synonyms

Reprogramming method

Vector type Non-integrating
Vector Episomal

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

Medium Other medium:
Base medium: Nuwacell ncTarget hPSC Medium
Main protein source:
Serum concentration: %

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-4
Yes
TRA 1-81
Yes
POU5F1 (OCT-4)
Yes
NANOG
Yes
Marker Present Absent
mCpG
OCT4 X
Self-renewal
Positive
Endoderm
Positive
Mesoderm
Positive
Ectoderm score
Positive
Differentiation Potency

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX

Other Genotyping (Cell Line)