General

Cell Line
hPSCreg name	VUBe020-A
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	VUBe020-A (RRID:CVCL_9855)
Alternative name(s)	VUB20_CMT1A
Cell line type	Human embryonic stem cell (hESC)
Similar lines	INSRMe015-A (STR-I-315-CMT1a) Donor diseases: Charcot-Marie-Tooth disease type 1
Last update	27th April 2022
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Provider
Generator	Vrije Universiteit Brussel (VUB) Contact: Department of Embryology and Genetics
Derivation country	Belgium
External Databases
Cellosaurus	CVCL_9855
Wikidata	Q54993406
General Information
Publications	Geens M et al. Human embryonic stem cell lines derived from single blastomeres of two 4-cell stage embryos. Human reproduction (Oxford, England). 2009 Nov;24(11):2709-17. Mateizel I et al. Derivation, culture, and characterization of VUB hESC lines. In vitro cellular & developmental biology. Animal. 2010 Apr;46(3-4):300-8.
* Is the cell line readily obtainable for third parties?	Yes

Donor Information

General Donor Information
Sex	female
Phenotype and Disease related information (Donor)
Diseases	A disease was diagnosed. Charcot-Marie-Tooth disease type 1 The donor is affected. Synonyms hereditary motor and sensory neuropathy type 1

Ethics

Alternatives to consent are available?	Yes
Alternatives to consent
Alternative consent approval number
How may genetic information associated with the cell line be accessed?
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions?	Yes
Name of accrediting authority involved?	Federal Commission Medical and Scientific Research on embryos in vitro (Belgium)
Approval number

hESC Derivation

Date of derivation	2007-07-25
Embryo stage	Blastula with ICM and Trophoblast
PGD Embryo?	Yes
ZP removal technique	Spontaneous Hatching
Cell isolation	Mechanical
Cell seeding	Isolated ICM

Culture Conditions

Feeder cells	mouse embryonic fibroblast cell Cellfinder Ont Id: EFO_0004040
O2 Concentration	20 %
CO2 Concentration	10 %
Medium	Other medium: Base medium: Knockout-DMEM Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements Supplement Amount Unit L-Glutamine

Characterisation

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles
Alkaline Phosphatase	Yes
TRA 1-60	Yes
TRA 1-81	Yes
DNMT3B	Yes
GDF3	Yes
NANOG	Yes
POU5F1 (OCT-4)	Yes
ZFP42 (REX-1)	Yes
SOX2	Yes

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes 46XX Passage number: 18
Other Genotyping (Cell Line)