INSRMe015-A

STR-I-315-CMT1a

General#

Cell Line

hPSCreg Name INSRMe015-A
Alternative name(s)
STR-I-315-CMT1a
Cell line type Human embryonic stem cell (hESC)
Last update 28th July 2017
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Provider

Generator INSERM (INSRM)
Derivation country France

External Databases

Cellosaurus CVCL_C095

General Information

Is the cell line available in principle?
Yes

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Charcot-Marie-Tooth disease type 1
The donor is affected.
Synonyms
  • hereditary motor and sensory neuropathy type 1
Disease associated phenotypes
  • Charcot-Marie Tooth type 1A

Ethics#

We are currently revising this section.

hESC Derivation#

Embryo stage Blastula with ICM and Trophoblast
PGD Embryo?
Yes
ZP removal technique Enzymatic

Culture Conditions#

Surface coating Gelatin
Passage method Mechanically
CO2 Concentration 5 %
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
L-Glutamine
bFGF

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
DNMT3B
Yes
GABRB3
Yes
GDF3
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
TDGF1
Yes
SSEA-1
No
SSEA-4
Yes
TRA 1-81
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY

Other Genotyping (Cell Line)