Derivation, culture, and characterization of VUB hESC lines


In this report, we present the derivation and characterization of 15 hESC lines established at the Vrije Universiteit Brussel, Belgium in collaboration with the Universitair Ziekenhuis Brussel, Belgium, using surplus in vitro fertilization embryos and embryos carrying monogenic disorders donated for research. Four lines were derived from blastocyst-stage embryos presumed to be genetically normal, and 11 hESC lines were obtained from embryos shown to carry genetic mutations by preimplantation genetic diagnosis. All the lines express markers of pluripotency as determined by immunocytochemistry and RT-PCR, and formed teratomas when injected into SCID mice. All VUB hESC lines, except for VUB17, are reported in the European hESC registry and are available upon request after signing a Material Transfer Agreement from the VUB (contact person: Prof. Dr. Karen Sermon;

Authors Mateizel I, Spits C, De Rycke M, Liebaers I, Sermon K
Journal In vitro cellular & developmental biology. Animal
Publication Date 2010 Apr;46(3-4):300-8
PubMed 20224973
DOI 10.1007/s11626-010-9284-4

Research Projects

Cell Lines