VUB11_FXS

General#

Cell Line

hPSCreg Name VUBe011-A
Alternative name(s)
VUB11_FXS
Cell line type Human embryonic stem cell (hESC)
Last update 27th October 2017
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Provider

Generator Vrije Universiteit Brussel (VUB)
Derivation country Belgium

External Databases

Cellosaurus CVCL_9848

General Information

Publications
Is the cell line available in principle?
Yes

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Fragile X syndrome
The donor is affected.
Synonyms
  • FXS
  • FraX syndrome
  • Martin-Bell syndrome
  • FRAXA syndrome
show more synonyms

Ethics#

Was the embryo established purely for research purposes? No
Have both parents consented to the use of the embryo for ESC derivation? Yes
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Alternatives to consent are available? Yes
Alternatives to consent
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Federal Commission Medical and Scientific Research on embryos in vitro (Belgium)
Approval number

hESC Derivation#

Date of derivation 2006-09-19
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
No
Other hESC source: IVF
PGD Embryo?
Yes
Cell isolation Immunosurgery
Cell seeding Isolated ICM

Culture Conditions#

Surface coating MEF
Feeder cells mouse embryonic fibroblast cell
Cellfinder Ont Id: EFO_0004040
O2 Concentration 20 %
CO2 Concentration 10 %
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
L-Glutamine
bFGF

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
SSEA-1
No
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
DNMT3B
Yes
GDF3
Yes
LIN28
Yes
NANOG
Yes
NPM1
Yes
POU5F1 (OCT-4)
Yes
ZFP42 (REX-1)
Yes
SOX2
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX

Other Genotyping (Cell Line)